pSEUDO, a Genetic Integration Standard for Lactococcus lactis

被引：30
作者：
Pinto, Joao P. C. [1 ]
Zeyniyev, Araz [1 ]
Karsens, Harma [1 ]
Trip, Hein [2 ]
Lolkema, Juke S. [2 ]
Kuipers, Oscar P. [1 ]
Kok, Jan [1 ]
机构：
[1] Univ Groningen, Dept Mol Genet, Groningen Biomol Sci & Biotechnol Inst, NL-9747 AG Groningen, Netherlands
[2] Univ Groningen, Dept Mol Microbiol, Groningen Biomol Sci & Biotechnol Inst, NL-9747 AG Groningen, Netherlands
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 18期
关键词：
COMPLETE GENOME SEQUENCE; OROTATE TRANSPORTER; ACID BACTERIA; EXPRESSION; CONSTRUCTION; MEMBRANE; CREMORIS; OROP;
D O I：
10.1128/AEM.05196-11
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Plasmid pSEUDO and derivatives were used to show that llmg_pseudo_10 in Lactococcus lactis MG1363 and its homologous locus in L. lactis IL1403 are suitable for chromosomal integrations. L. lactis MG1363 and IL1403 nisin-induced controlled expression (NICE) system derivatives (JP9000 and IL9000) and two general stress reporter strains (NZ9000::PhrcA-GFP and NZ9000::PgroES-GFP) enabling in vivo noninvasive monitoring of cellular fitness were constructed.
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收藏
页码：6687 / 6690
页数：4
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