Adenovirus-mediated human brain-derived neurotrophic factor gene-modified bone marrow mesenchymal stem cell transplantation for spinal cord injury

被引：0
作者：
Wang, Changsheng [1 ]
Lin, Jianhua [1 ]
Wu, Chaoyang [1 ]
Chen, Rongsheng [1 ]
机构：
[1] Fujian Med Univ, Affiliated Hosp 1, Dept Spinal Surg, Fuzhou 350005, Fujian Province, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2011年 / 6卷 / 16期
基金：
高等学校博士学科点专项科研基金;
关键词：
brain-derived neurotrophic factor; bone marrow mesenchymal stem cells; gene modification; intravenous transplantation; spinal cord injury; neural regeneration; NEURONS;
D O I：
10.3969/j.issn.1673-5374.2011.16.002
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Rat bone marrow mesenchymal stem cells expressing brain-derived neurotrophic factor were successfully obtained using a gene transfection method, then intravenously transplanted into rats with spinal cord injury. At 1, 3, and 5 weeks after transplantation, the expression of brain-derived neurotrophic factor and neurofilament-200 was upregulated in the injured spinal cord, spinal cord injury was alleviated, and Basso-Beattie-Bresnahan scores of hindlimb motor function were significantly increased. This evidence suggested that intravenous transplantation of adenovirus-mediated brain-derived neurotrophic factor gene-modified rat bone marrow mesenchymal stem cells could play a dual role, simultaneously providing neural stem cells and neurotrophic factors.
引用
收藏
页码：1211 / 1216
页数：6
相关论文
共 17 条
  • [1] MASCIS evaluation of open field locomotor scores: Effects of experience and teamwork on reliability
    Basso, DM
    Beattie, MS
    Bresnahan, JC
    Anderson, DK
    Faden, AI
    Gruner, JA
    Holford, TR
    Hsu, CY
    Noble, LJ
    Nockels, R
    Perot, PL
    Salzman, SK
    Young, W
    [J]. JOURNAL OF NEUROTRAUMA, 1996, 13 (07) : 343 - 359
  • [2] Human mesenchymal stem cells express neural genes, suggesting a neural predisposition
    Blondheim, NR
    Levy, YS
    Ben-Zur, T
    Burshtein, A
    Cherlow, T
    Kan, I
    Barzilai, R
    Bahat-Stromza, M
    Barhum, Y
    Bulvik, S
    Melamed, E
    Offen, D
    [J]. STEM CELLS AND DEVELOPMENT, 2006, 15 (02) : 141 - 164
  • [3] CHEN D, 2007, SHENJING JIEPOU XUE, V23, P635
  • [4] Mesenchymal stem cells: Biology and potential clinical uses
    Deans, RJ
    Moseley, AB
    [J]. EXPERIMENTAL HEMATOLOGY, 2000, 28 (08) : 875 - 884
  • [5] Mesenchymal stem cells spontaneously express neural proteins in culture and are neurogenic after transplantation
    Deng, Jie
    Petersen, Bryon E.
    Steindler, Dennis A.
    Jorgensen, Marda L.
    Laywell, Eric D.
    [J]. STEM CELLS, 2006, 24 (04) : 1054 - 1064
  • [6] DONG F, 2008, ZHONGGUO KANGFU YIXU, V23, P416
  • [7] LIN JH, 2005, ZHONGHUA GUKE ZAZHI, V25, P556
  • [8] Lin Jianhua, 2010, Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi, V24, P982
  • [9] BDNF in sensory neurons and chronic pain
    Obata, Koichi
    Noguchi, Koichi
    [J]. NEUROSCIENCE RESEARCH, 2006, 55 (01) : 1 - 10
  • [10] The Ministry of Science and Technology of the People's Republic of China, 2006, GUID SUGG CAR US LAB
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