High energy shock waves (HESW) increase paclitaxel efficacy in a syngeneic model of breast cancer

被引：13

作者：

Canaparo, Roberto ^{[1
,2
]}

Serpe, Loredana ^{[2
]}

Zara, Gian Paolo ^{[2
]}

Chiarle, Roberto ^{[3
,4
]}

Berta, Laura ^{[1
]}

Frairia, Roberto ^{[1
]}

机构：

[1] Univ Turin, Dept Clin Pathophysiol, I-10126 Turin, Italy

[2] Univ Turin, Dept Anat Pharmacol & Forens Med, I-10125 Turin, Italy

[3] Univ Turin, Dept Pathol, I-10126 Turin, Italy

[4] Univ Turin, CeRMS, I-10126 Turin, Italy

来源：

TECHNOLOGY IN CANCER RESEARCH & TREATMENT | 2008年 / 7卷 / 02期

关键词：

high energy shock waves (HESW); paclitaxel; breast cancer; Fisher; 344; rats; Mat B-III cells;

D O I：

10.1177/153303460800700204

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The combined effect of high energy shock waves (HESW), generated by a piezoelectric device, and paclitaxel on Mat B-III rat breast cancer cells in vitro and in an in vivo animal model is presented. A significant reduction of in vitro Mat B-III cell proliferation versus cells treated with paclitaxel alone was observed with the combined exposure to paclitaxel (0.1, 1, or 10 nM) and HESW (0.22 mJ/mm(2), 1000 shots). Moreover earlier induction and enhanced apoptosis occurred in cells subjected to the combined treatment with paclitaxel and HESW at 1 and 10 nM versus paclitaxel alone (p<0.01 and p<0.001, respectively). The percentage of apoptotic cells along with BAD mRNA expression, confirm a significant enhancement of apoptosis in tumor tissues subjected to the combined treatment with paclitaxel (2.5 mg/kg on days 7 and 11) and HESW (0.50 mJ/mm(2), 500 shots on day 11) in comparison with paclitaxel alone. In conclusion, these data suggest that HESW enhance paclitaxel cytotoxicity in the Mat B-III syngeneic model of breast cancer.

引用

页码：117 / 124

页数：8

共 29 条

[1] Taxol-induced apoptosis depends on MAP kinase pathways (ERK and p38) and is independent of p53 [J].