DNA damage and NF-κB inactivation implicate glycyrrhizic acid-induced G1 phase arrest in hepatocellular carcinoma cells

被引：18
作者：
Wang, Wei-Shu [1 ,2 ]
Chen, Yu-Shan [3 ]
Kuo, Chen-Yu [4 ]
Tsai, Jai-Jen [2 ,4 ,5 ]
Hsu, Fei-Ting [6 ]
Chung, Jing-Gung [6 ,7 ]
Pan, Po-Jung [2 ,8 ]
机构：
[1] Natl Yang Ming Chiao Tung Univ Hosp, Dept Med, Yilan, Taiwan
[2] Natl Yang Ming Chiao Tung Univ, Sch Med, Taipei, Taiwan
[3] Show Chwan Mem Hosp, Dept Radiat Oncol, Changhua, Taiwan
[4] Natl Yang Ming Chiao Tung Univ Hosp, Dept Med, Div Gastroenterol, Yilan, Taiwan
[5] Cardinal Tien Jr Coll Healthcare & Management, Dept Nursing, New Taipei, Taiwan
[6] China Med Univ, Dept Biol Sci & Technol, Taichung, Taiwan
[7] Asia Univ, Dept Med Lab & Biotechnol, Taichung, Taiwan
[8] Natl Yang Ming Chiao Tung Univ Hosp, Dept Phys Med & Rehabil, Yilan, Taiwan
来源：
JOURNAL OF FOOD BIOCHEMISTRY | 2022年 / 46卷 / 07期
关键词：
DNA damage; G1; arrest; glycyrrhizic acid; hepatocellular carcinoma cells; NF-kappa B; CYCLE ARREST; CANCER CELLS; APOPTOSIS; PROLIFERATION; P21;
D O I：
10.1111/jfbc.14128
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hepatocellular carcinoma (HCC) is the most frequently occurring liver malignancy in Asia. Glycyrrhizic acid is known to reduce the risk of HCC formation in patients with chronic hepatitis C. To identify whether glycyrrhizic acid may play a role in anti-HCC therapy as an adjuvant is important. However, the inhibitory effect of glycyrrhizic acid on cell cycle progression in HCC cells and the mechanism of such have not been fully elucidated. This study used the comet assay, cell cycle analysis, immunofluorescence staining, the TUNEL assay, and Western blotting to identify the anti-HCC role of glycyrrhizic acid. Glycyrrhizic acid may induce DNA damage, apoptosis, activation of ATM, and expression of p21, and p27 in HCC cells. In addition, glycyrrhizic acid may also induce G(1) phase arrest and suppress NF-kappa B-mediated Cyclin D1 expression. DNA damage and NF-kappa B inactivation may be associated with glycyrrhizic acid-induced G(1) phase arrest in HCC cells.
引用
收藏
页数：8
相关论文
共 40 条
[1]  
Abo ElMagd N. F., 2015, Egyptian Journal of Basic and Applied Sciences, V2, P65
[2]  
Arase Y, 1997, CANCER, V79, P1494, DOI 10.1002/(SICI)1097-0142(19970415)79:8<1494::AID-CNCR8>3.0.CO
[3]  
2-B
[4]   DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation [J].
Bakkenist, CJ ;
Kastan, MB .
NATURE, 2003, 421 (6922) :499-506
[5]   Baicalein: A review of its anti-cancer effects and mechanisms in Hepatocellular Carcinoma [J].
Bie, Beibei ;
Sun, Jin ;
Guo, Ying ;
Li, Jun ;
Jiang, Wei ;
Yang, Jun ;
Huang, Chen ;
Li, Zongfang .
BIOMEDICINE & PHARMACOTHERAPY, 2017, 93 :1285-1291
[6]   Glycyrrhizic Acid-Induced Differentiation Repressed Stemness in Hepatocellular Carcinoma by Targeting c-Jun N-Terminal Kinase 1 [J].
Cai, Shijiao ;
Bi, Zhun ;
Bai, Yunpeng ;
Zhang, Heng ;
Zhai, Denghui ;
Xiao, Cui ;
Tang, Yuanhao ;
Yang, Lan ;
Zhang, Xiaoyun ;
Li, Kun ;
Yang, Ru ;
Liu, Yanrong ;
Chen, Shuang ;
Sun, Tao ;
Liu, Huijuan ;
Yang, Cheng .
FRONTIERS IN ONCOLOGY, 2020, 9
[7]   Glycyrrhizin Acid and Glycyrrhetinic Acid Modified Polyethyleneimine for Targeted DNA Delivery to Hepatocellular Carcinoma [J].
Cao, Mingzhuo ;
Gao, Yong ;
Zhan, Mengling ;
Qiu, Nasha ;
Piao, Ying ;
Zhou, Zhuxian ;
Shen, Youqing .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2019, 20 (20)
[8]   Induction of Apoptosis, Inhibition of MCL-1, and VEGF-A Expression Are Associated with the Anti-Cancer Efficacy of Magnolol Combined with Regorafenib in Hepatocellular Carcinoma [J].
Chen, Cheng-Hsien ;
Hsu, Fei-Ting ;
Chen, Wei-Lung ;
Chen, Jiann-Hwa .
CANCERS, 2021, 13 (09)
[9]   New roles for p21 and p27 cell-cycle inhibitors: a function for each cell compartment? [J].
Coqueret, O .
TRENDS IN CELL BIOLOGY, 2003, 13 (02) :65-70
[10]   DNA damage-induced cell cycle checkpoints and DNA strand break repair in development and tumorigenesis [J].
Dasika, GK ;
Lin, SCJ ;
Zhao, S ;
Sung, P ;
Tomkinson, A ;
Lee, EYHP .
ONCOGENE, 1999, 18 (55) :7883-7899
1234