Myostatin gene silenced by RNAi show a zebrafish giant phenotype

被引：182

作者：

Acosta, J ^{[1
]}

Carpio, Y ^{[1
]}

Borroto, I ^{[1
]}

González, O ^{[1
]}

Estrada, MP ^{[1
]}

机构：

[1] Ctr Genet Engn & Biotechnol, Anim Biotechnol Div, Aquat Biotechnol Project, Havana 10600, Cuba

来源：

JOURNAL OF BIOTECHNOLOGY | 2005年 / 119卷 / 04期

关键词：

zebrafish myostatin; RNAi gene silencing; hypertrophy; hyperplasia;

D O I：

10.1016/j.jbiotec.2005.04.023

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Myostatin is a member of the transforming growth factor-beta (TGF-beta) family that functions as, a negative regulator of skeletal muscle development and growth. Recently, it has been reported that the transgenic zebrafish expressing myostatin prodomain exhibited an increased number of fiber in skeletal muscle. Other novel results suggest that myostatin plays a mayor role during myogenesis, apart from inhibition of proliferation as well as differentiation, We have investigated the ability of double-stranded RNA (dsRNA) to inhibit myostatin function in the zebrafish. By microinjection dsRNA, corresponding to biologically active C-terminal domain from aminoacid 268 to end codon of tilapia myostatin protein. we produced an increased body mass in treated fish. The dsRNA injection in early development stage in zebrafish produced hyperplasia or hypertrophy. In addition, the interference of gene function showed a strong dependence on the amount of dsRNA. (C) 2005 Elsevier B.V. All rights reserved.

引用

页码：324 / 331

页数：8

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