Acute effects of brain-derived neurotrophic factor on energy expenditure in obese diabetic mice

被引:69
|
作者
Tsuchida, A
Nonomura, T
Ono-Kishino, M
Nakagawa, T
Taiji, M
Noguchi, H
机构
[1] Sumitomo Pharmaceut Co Ltd, Discovery Res Labs 2, Konohana Ku, Osaka 5540022, Japan
[2] Sumitomo Pharmaceut Co Ltd, Business Dev & Licensing Off, Tokyo, Japan
关键词
neurotrophic factor; thermogenesis; glucose metabolism; norepinephrine turnover; uncoupling protein;
D O I
10.1038/sj.ijo.0801678
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
OBJECTIVE: We recently demonstrated that chronic treatment with brain-derived neurotrophic factor (BDNF) regulates energy expenditure in obese diabetic C57BL/KsJ-db/db mice. In this study, we investigated the acute effects of BDNF on energy expenditure. DESIGN: After BDNF was singly administered to male db/db mice (aged 10-12 weeks), their body temperature and whole body glucose oxidation were measured. Their norepinephrine (NE) turnover and uncoupling protein (UCP) 1 expression in interscapular brown adipose tissue (BAT) were also analyzed. RESULTS: Even though the body temperatures of hyperphagic db/db mice dropped remarkably in a 24 h period after food deprivation, only a single subcutaneous administration of BDNF significantly prevented the reduction of body temperature. BDNF was also observed to have similar efficacy in cold exposure experiments at 15 degreesC. Respiratory excretion Of (CO2)-C-14 after intravenous injection of D-[(14C)(U)]-glucose was significantly increased by BDNF administration, indicating that BDNF increases whole-body glucose oxidation. BDNF administered intracerebroventricularly was also able to prevent the reduction of body temperature of db/db mice. To clarify the BDNF action mechanism we examined NE turnover in BAT. Four hours after a single administration, BDNF reduced NE content in the presence of the tyrosine hydroxylase inhibitor, alpha -methyl-P-tyrosine methyl ester, indicating enhanced NE turnover in BAT. BDNF also increased the expression of the UCP1 mRNA and protein in BAT. CONCLUSION: These data indicate that BDNF rapidly regulates energy metabolism in obese diabetic animals, partly through activating the sympathetic nervous system and inducing UCP1 gene expression in BAT.
引用
收藏
页码:1286 / 1293
页数:8
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