Bioprospecting and characterization of pigmented halophilic archaeal strains from Algerian hypersaline environments with analysis of carotenoids produced by Halorubrum sp. BS2

被引：27

作者：

Sahli, Kaouther ^{[1
]}

Gomri, Mohamed A. ^{[1
]}

Esclapez, Julia ^{[2
]}

Gomez-Villegas, Patricia ^{[3
]}

Ghennai, Oulaya ^{[1
]}

Bonete, Maria-Jose ^{[2
]}

Leon, Rosa ^{[3
]}

Kharroub, Karima ^{[1
]}

机构：

[1] Univ Freres Mentouri Constantine 1 UFMC1, Inst Nutr Alimentat & Technol Agroalimentaires IN, Lab Rech Biotechnol Qualite Aliments BIOQUAL, Constantine, Algeria

[2] Univ Alicante, Biochem & Mol Biol Div, Agrochem & Biochem Dept, Fac Sci, Alicante, Spain

[3] Univ Huelva, Fac Expt Sci, Biochem Lab, Marine Int Campus Excellence CEIMAR, Huelva, Spain

来源：

JOURNAL OF BASIC MICROBIOLOGY | 2020年 / 60卷 / 07期

关键词：

Algeria; antibacterial; antioxidant; carotenoids; haloarchaea; DIVERSITY; IDENTIFICATION; HALOARCHAEA;

D O I：

10.1002/jobm.202000083

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A set of 110 extremely halophilic archaeal strains were isolated from seven distinct saline habitats located in different regions of Algeria. The physicochemical characterization of the samples showed that these habitats were thalassohaline. The carotenoid production from isolated strains varied from 0.1 to 3.68 mu g/ml. Based on their physiological characteristics and pigment production, 43 strains were selected and identified by means of phenotypic tests and 16S ribosomal RNA gene sequencing. Phylogenetic analysis indicated that the isolates corresponded to the class Halobacteria and were closely related to genera Halorubrum, Haloarcula, Haloferax, Natrinema, Halogeometricum, Haloterrigena, and Halopiger. Carotenoids of the highest producer, strain Halorubrum sp. BS2 were identified using high-performance liquid chromatography-diode array detector and liquid chromatography-mass spectrometry. Bacterioruberin and bisanhydrobacterioruberin were the predominant carotenoids. The scavenging activity of these carotenoids reached 99% at a concentration of 18 mu g/ml, which was much higher than that of ascorbic acid used as a reference compound. These carotenoids also exhibited significant antibacterial activities against four human-pathogenic strains and four fish-pathogenic strains. Variations in salinity, agitation rate, temperature, and light intensity were found to influence growth and carotenoid production of Halorubrum sp. BS2. Our results suggest that halophilic archaea represent a potential source for carotenoids, which are characterized by high antioxidant and antibacterial activities.

引用

页码：624 / 638

页数：15