Overexpression of MdZAT5, an C2H2-Type Zinc Finger Protein, Regulates Anthocyanin Accumulation and Salt Stress Response in Apple Calli and Arabidopsis

被引：51

作者：

Wang, Da-Ru ^{[1
]}

Yang, Kuo ^{[1
]}

Wang, Xun ^{[1
]}

Lin, Xiao-Lu ^{[2
]}

Rui, Lin ^{[1
]}

Liu, Hao-Feng ^{[1
]}

Liu, Dan-Dan ^{[3
]}

You, Chun-Xiang ^{[1
]}

机构：

[1] Shandong Agr Univ, Natl Key Lab Crop Biol, Natl Res Ctr Apple Engn & Technol, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China

[2] Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China

[3] Yunnan Univ, Coll Agr, Kunming 650091, Yunnan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 03期

基金：

中国国家自然科学基金;

关键词：

apple; MdZAT5; anthocyanin; salt tolerance; MYB TRANSCRIPTION FACTOR; ROOT HAIR DEVELOPMENT; DROUGHT TOLERANCE; ABIOTIC STRESS; POSITIVE REGULATOR; GENE; LIGHT; TEMPERATURE; RESISTANCE; MECHANISM;

D O I：

10.3390/ijms23031897

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc finger proteins are widely involved and play an important role in plant growth and abiotic stress. In this research, MdZAT5, a gene encoding C2H2-type zinc finger protein, was cloned and investigated. The MdZAT5 was highly expressed in flower tissues by qRT-PCR analyses and GUS staining. Promoter analysis showed that MdZAT5 contained multiple response elements, and the expression levels of MdZAT5 were induced by various abiotic stress treatments. Overexpression of MdZAT5 in apple calli positively regulated anthocyanin accumulation by activating the expressions of anthocyanin biosynthesis-related genes. Overexpression of MdZAT5 in Arabidopsis also enhanced the accumulation of anthocyanin. In addition, MdZAT5 increased the sensitivity to salt stress in apple calli. Ectopic expression of MdZAT5 in Arabidopsis reduced the expression of salt-stress-related genes (AtNHX1 and AtABI1) and improved the sensitivity to salt stress. In conclusion, these results suggest that MdZAT5 plays a positive regulatory role in anthocyanin accumulation and negatively regulates salt resistance.

引用

页数：16

共 76 条

[1] Role of DREB transcription factors in abiotic and biotic stress tolerance in plants [J].