PCR engineering of viral quasispecies: A new method to preserve and manipulate genetic diversity of RNA virus populations

被引：22
作者：
Chumakov, KM
机构：
来源：
JOURNAL OF VIROLOGY | 1996年 / 70卷 / 10期
关键词：
D O I：
10.1128/JVI.70.10.7331-7334.1996
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A PCR-based method for the controlled manipulation of individual genomic sites of poliovirus with concomitant preservation of the sequence heterogeneity of the rest of the genome is proposed. The new approach can be used for the creation of stable DNA repositories of populations of extremely heterogenous RNA viruses and may have implications for live vaccine technology.
引用
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页码：7331 / 7334
页数：4
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