Poly(β-amino ester) and cationic phospholipid-based lipopolyplexes for gene delivery and transfection in human aortic endothelial and smooth muscle cells

被引：27

作者：

Brito, Luis

Little, Steven ^{[2
]}

Langer, Robert ^{[3
]}

Amiji, Mansoor ^{[1
]}

机构：

[1] Northeastern Univ, Dept Pharmaceut Sci, Boston, MA 02115 USA

[2] Univ Pittsburgh, Dept Chem Engn, Pittsburgh, PA 15261 USA

[3] MIT, Dept Chem Engn, Cambridge, MA 02139 USA

来源：

BIOMACROMOLECULES | 2008年 / 9卷 / 04期

关键词：

D O I：

10.1021/bm7011373

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Safe and effective nonviral gene delivery and transfection in primary human vascular endothelial cells (EC) and smooth muscle cells (SMC) has tremendous potential for cardiovascular diseases such as in the treatment of coronary restenosis. Using a combination of a cationic biodegradable polymer, poly(beta-amino ester) (PBAE), and a cationic phospholipid, 1,2-dioleoyl-3-trimethylammonium propane (DOTAP), we have engineered a lipopolyplex nanovector system that can transfect EC and SMC cells with reasonably high efficiency. For instance, upon addition of 1.0 mu g DNA complexed in lipopolyplexes the transfection efficiency in SMC was 20% and in EC was 33%. The results of this study shows that PBAE-DOTAP-plasmid DNA lipopolyplexes are a promising nonviral vector system for gene delivery and transfection in EC and SMC.

引用

页码：1179 / 1187

页数：9

共 35 条

[1] Synthesis of poly(β-amino ester)s optimized for highly effective gene delivery [J].