Isolation of an antioxidant peptide from krill protein hydrolysates as a novel agent with potential hepatoprotective effects

被引:29
作者
Fernando, Ilekuttige Priyan Shanura [1 ]
Park, Soo Yeon [1 ]
Han, Eui Jeong [2 ]
Kim, Hyun-Soo [3 ]
Kang, Dong-Soo [1 ]
Je, Jae-Young [4 ]
Ahn, Chang-Bum [2 ]
Ahn, Ginnae [1 ,2 ]
机构
[1] Chonnam Natl Univ, Dept Marine Biofood Sci, Yeosu 59626, South Korea
[2] Chonnam Natl Univ, Dept Food Technol & Nutr, Yeosu 59626, South Korea
[3] Natl Marine Biodevers Inst Korea, 75 Jangsan Ro 101 Gil, Seocheon, South Korea
[4] Pukyong Natl Univ, Dept Marine Bio Convergence Sci, Busan 48547, South Korea
基金
新加坡国家研究基金会;
关键词
Euphausia superba; Bioactive peptide; Hepatoprotective effects; Oxidative stress; Protein hydrolysate; REGULATES HEME OXYGENASE-1; NF-E2-RELATED FACTOR-2; EUPHAUSIA-SUPERBA; OXIDATIVE STRESS; CELLS; PURIFICATION; ACTIVATION; LIVER; INFLAMMATION; GENERATION;
D O I
10.1016/j.jff.2020.103889
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Krill accounts for the highest abundant animal biomass on earth though it remains underexplored. During the present study, krill protein hydrolysates (KPH) were prepared using several proteases, optimizing reaction time for dose-response analysis of antioxidant activity. Bioassay-guided fractionation of selected KPH of pepsin, first by ultrafiltration ( < 1 kDa, 1-3 kDa, and > 3 kDa), revealed the active fraction, 1-3 kDa, which was consecutively separated by ion-exchange chromatography and stepwise RP-HPLC. Three peptides were identified, sequenced and synthesized. The peptide P2 was effective in reducing H2O2-induced oxidative stress and lipid peroxidation by increasing antioxidant enzyme activities, including superoxide dismutase, catalase, and glutathione peroxidase. P2 reduced apoptosis in H2O2 -stimulated hepatocytes by regulating the expression levels of Bcl-2/Bax and caspase-3. P2 pre-treatment increased nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) expression in cultured hepatocytes. Furthermore, P2 induced the activation of Nrf2/HO-1 by activating the ERK pathway.
引用
收藏
页数:9
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