Krill accounts for the highest abundant animal biomass on earth though it remains underexplored. During the present study, krill protein hydrolysates (KPH) were prepared using several proteases, optimizing reaction time for dose-response analysis of antioxidant activity. Bioassay-guided fractionation of selected KPH of pepsin, first by ultrafiltration ( < 1 kDa, 1-3 kDa, and > 3 kDa), revealed the active fraction, 1-3 kDa, which was consecutively separated by ion-exchange chromatography and stepwise RP-HPLC. Three peptides were identified, sequenced and synthesized. The peptide P2 was effective in reducing H2O2-induced oxidative stress and lipid peroxidation by increasing antioxidant enzyme activities, including superoxide dismutase, catalase, and glutathione peroxidase. P2 reduced apoptosis in H2O2 -stimulated hepatocytes by regulating the expression levels of Bcl-2/Bax and caspase-3. P2 pre-treatment increased nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase 1 (HO-1) expression in cultured hepatocytes. Furthermore, P2 induced the activation of Nrf2/HO-1 by activating the ERK pathway.
机构:
Seoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South KoreaSeoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South Korea
Chen, CY
;
Jang, JH
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Seoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South KoreaSeoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South Korea
Jang, JH
;
Li, MH
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h-index: 0
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Seoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South KoreaSeoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South Korea
Li, MH
;
Surh, YJ
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h-index: 0
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Seoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South KoreaSeoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South Korea
机构:
E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R ChinaShanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R China
Chen, Ming-cang
;
Ye, Yi-yi
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机构:
E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R ChinaShanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R China
Ye, Yi-yi
;
Ji, Guang
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h-index: 0
机构:
Shanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R ChinaShanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R China
Ji, Guang
;
Liu, Jian-wen
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h-index: 0
机构:
E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R ChinaShanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R China
机构:
Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Fernando, I. P. Shanura
;
Jayawardena, Thilina U.
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h-index: 0
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Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Jayawardena, Thilina U.
;
Kim, Hyun-Soo
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Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Kim, Hyun-Soo
;
Lee, Won Woo
论文数: 0引用数: 0
h-index: 0
机构:
Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Nakdonggang Natl Inst Biol Resources, Freshwater Bioresources Utilizat Div, Sangju 37242, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Lee, Won Woo
;
Vaas, A. P. J. P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colombo, Dept Chem, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Vaas, A. P. J. P.
;
De Silva, H. I. C.
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Univ Colombo, Dept Chem, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
De Silva, H. I. C.
;
Abayaweera, G. S.
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Univ Colombo, Dept Chem, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Abayaweera, G. S.
;
Nanayakkara, C. M.
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Univ Colombo, Dept Plant Sci, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Nanayakkara, C. M.
;
Abeytunga, D. T. U.
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Univ Colombo, Dept Chem, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Abeytunga, D. T. U.
;
Lee, Dae-Sung
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h-index: 0
机构:
Natl Marine Biodevers Inst Korea, Dept Appl Res, 75 Jangsan Ro 101 Gil, Seocheon, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Lee, Dae-Sung
;
Jeon, You-Jin
论文数: 0引用数: 0
h-index: 0
机构:
Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
机构:
Seoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South KoreaSeoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South Korea
Chen, CY
;
Jang, JH
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h-index: 0
机构:
Seoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South KoreaSeoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South Korea
Jang, JH
;
Li, MH
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South KoreaSeoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South Korea
Li, MH
;
Surh, YJ
论文数: 0引用数: 0
h-index: 0
机构:
Seoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South KoreaSeoul Natl Univ, Coll Pharm, Natl Res Lab Mol Carcinogenesis & Chemoprevent, Seoul 151742, South Korea
机构:
E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R ChinaShanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R China
Chen, Ming-cang
;
Ye, Yi-yi
论文数: 0引用数: 0
h-index: 0
机构:
E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R ChinaShanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R China
Ye, Yi-yi
;
Ji, Guang
论文数: 0引用数: 0
h-index: 0
机构:
Shanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R ChinaShanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R China
Ji, Guang
;
Liu, Jian-wen
论文数: 0引用数: 0
h-index: 0
机构:
E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R ChinaShanghai Univ Tradit Chinese Med, Longhua Hosp, Lab Liver Dis, Shanghai, Peoples R China
机构:
Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Fernando, I. P. Shanura
;
Jayawardena, Thilina U.
论文数: 0引用数: 0
h-index: 0
机构:
Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Jayawardena, Thilina U.
;
Kim, Hyun-Soo
论文数: 0引用数: 0
h-index: 0
机构:
Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Kim, Hyun-Soo
;
Lee, Won Woo
论文数: 0引用数: 0
h-index: 0
机构:
Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Nakdonggang Natl Inst Biol Resources, Freshwater Bioresources Utilizat Div, Sangju 37242, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Lee, Won Woo
;
Vaas, A. P. J. P.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colombo, Dept Chem, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Vaas, A. P. J. P.
;
De Silva, H. I. C.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colombo, Dept Chem, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
De Silva, H. I. C.
;
Abayaweera, G. S.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colombo, Dept Chem, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Abayaweera, G. S.
;
Nanayakkara, C. M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colombo, Dept Plant Sci, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Nanayakkara, C. M.
;
Abeytunga, D. T. U.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Colombo, Dept Chem, Colombo 3, Sri LankaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Abeytunga, D. T. U.
;
Lee, Dae-Sung
论文数: 0引用数: 0
h-index: 0
机构:
Natl Marine Biodevers Inst Korea, Dept Appl Res, 75 Jangsan Ro 101 Gil, Seocheon, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea
Lee, Dae-Sung
;
Jeon, You-Jin
论文数: 0引用数: 0
h-index: 0
机构:
Jeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South KoreaJeju Natl Univ, Dept Marine Life Sci, Jeju 63243, South Korea