In Vivo and In Vitro Anticancer Activity of Doxorubicin-loaded DNA-AuNP Nanocarrier for the Ovarian Cancer Treatment

被引：28
作者：
Lee, Chang-Seuk [1 ]
Kim, Tae Wan [2 ]
Oh, Da Eun [1 ]
Bae, Su Ok [1 ]
Ryu, Jaesung [2 ]
Kong, Hyejeong [2 ]
Jeon, Hyeji [3 ]
Seo, Hee Kyung [3 ]
Jeon, Seob [3 ]
Kim, Tae Hyun [1 ]
机构：
[1] Soonchunhyang Univ, Dept Chem, Asan 31538, South Korea
[2] Soonchunhyang Univ, Dept Med Life Sci, Asan 31538, South Korea
[3] Soonchunhyang Univ, Cheonan Hosp, Dept Obstet & Gynecol, Coll Med, Cheonan 31151, South Korea
来源：
CANCERS | 2020年 / 12卷 / 03期
基金：
新加坡国家研究基金会;
关键词：
Nanomedicine; doxorubicin; gold nanoparticles; ovarian cancer; drug delivery; DRUG-DELIVERY SYSTEMS; GOLD-NANOPARTICLES; TARGETED DELIVERY; RELEASE; GRAPHENE;
D O I：
10.3390/cancers12030634
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
In this study, we have determined the anticancer activity of doxorubicin (Dox)-loaded DNA/gold nanoparticle (AuNP) nanocarrier (Dox-DNA-AuNP) for the treatment of ovarian cancer. The anticancer effect of Dox-DNA-AuNP was evaluated in vitro using the EZ-Cytox cell viability assay on three human ovarian cancer cell lines, SK-OV-3, HEY A8, and A2780. Dox-DNA-AuNP exhibited outstanding activity with good IC50 values of 4.8, 7.4, and 7.6 nM for SK-OV-3, HEY A8, and A2780, respectively. In vivo evaluation further demonstrated the superior anticancer effects of Dox-DNA-AuNP by inhibiting tumor growth compared to free Dox in an established SK-OV-3 xenograft mice model. Dox-DNA-AuNP showed about a 2.5 times higher tumor growth inhibition rate than free Dox. Furthermore, the immunohistochemical analysis of Ki67 antigen expression showed the lowest number of proliferative cells in the ovarian tumor tissue treated with Dox-DNA-AuNP. These results suggest Dox-DNA-AuNP might be a potential effective agent in ovarian cancer chemotherapy.
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页数：14
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