Gas chromatography tandem mass spectrometry offers advantages for urinary steroids analysis

被引：25

作者：

Homer, Natalie ^{[1
]}

Kothiya, Sanjay ^{[2
]}

Rutter, Alison ^{[2
]}

Walker, Brian R. ^{[2
]}

Andrew, Ruth ^{[2
]}

机构：

[1] Univ Edinburgh, Edinburgh Clin Res Facil, Queens Med Res Inst, Mass Spectrometry Core, 47,Little France Crescent, Edinburgh EH16 2TJ, Midlothian, Scotland

[2] Univ Edinburgh, Univ British Heart Fdn, Ctr Cardiovasc Sci, Queens Med Res Inst, 47,Little France Crescent, Edinburgh EH16 2TJ, Midlothian, Scotland

来源：

ANALYTICAL BIOCHEMISTRY | 2017年 / 538卷

基金：

英国惠康基金;

关键词：

Steroid; Triple quadrupole; Tandem mass spectrometry; Urine; Corticosteroid; Androgen;

D O I：

10.1016/j.ab.2017.09.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gas chromatography mass spectrometry has been the lynchpin of clinical assessment of steroid profiles for 3 decades. The improvements in assay performance offered by tandem mass spectrometry were assessed. Across the spectrum of glucocorticoid and androgen analytes tested, limits of detection and quantitation were similar to 20 fold lower with triple than single quadrupole systems, but the more noticeable improvement was that signal to noise was substantially improved and the linear range wider. These benefits allowed more reliable and concomitant measurement of steroids with substantially different abundances and in smaller volumes of urine. (C) 2017 The Author(s). Published by Elsevier Inc.

引用

页码：34 / 37

页数：4

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