Effect of electrical discharge plasma on cytotoxicity against cancer cells of N, O-carboxymethyl chitosan-stabilized gold nanoparticles

被引：14

作者：

Chokradjaroen, Chayanaphat ^{[1
]}

Rujiravanit, Ratana ^{[1
,2
]}

Theeramunkong, Sewan ^{[3
]}

Saito, Nagahiro ^{[4
]}

机构：

[1] Chulalongkorn Univ, Petr & Petrochem Coll, Bangkok 10330, Thailand

[2] Chulalongkorn Univ, Ctr Excellence Petrochem & Mat Technol, Bangkok 10330, Thailand

[3] Thammasat Univ, Fac Pharm, Pathum Thani 12120, Thailand

[4] Nagoya Univ, Grad Sch Engn, Dept Chem Syst Engn, Nagoya, Aichi 4648603, Japan

来源：

CARBOHYDRATE POLYMERS | 2020年 / 237卷

关键词：

Gold nanoparticles; N; O-carboxymethyl chitosan; Cytotoxicity; Cancer cells; Electrical discharge plasma; MOLECULAR-WEIGHT; CELLULAR UPTAKE; ALGINATE; CHITOOLIGOSACCHARIDES; PENETRATION; DEGRADATION; RESONANCE; DELIVERY; MATRIX; FATE;

D O I：

10.1016/j.carbpol.2020.116162

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Electrical discharge plasma in a liquid phase can generate reactive species, e.g. hydroxyl radical, leading to rapid reactions including degradation of biopolymers. In this study, the effect of plasma treatment time on physical properties and cytotoxicity against cancer cells of N,O-carboxymethyl chitosan-stabilized gold nanoparticles (CMC-AuNPs) was investigated. AuNPs were synthesized by chemical reduction of HAuCl4 in 2 % CMC solution to obtain CMC-AuNPs, before being subjected to the plasma treatment. Results showed that the plasma treatment not only led to the reduction of hydrodynamic diameters of CMC-AuNPs from 400 nm to less than 100 nm by the plasma-induced degradation of CMC but also provided the narrow size distribution of AuNPs having diameters in the range of 2-50 nm, that were existing in CMC-AuNPs. In addition, the plasma-treated CMC-AuNPs could significantly reduce the percentage of cell viability of breast cancer cells by approximately 80 % compared to the original CMC and CMC-AuNPs.

引用

页数：11