Recurrence monitoring for ovarian cancer using a cell phone-integrated paper device to measure the ovarian cancer biomarker HE4/CRE ratio in urine

被引:5
作者
Kight, Emily C. [1 ]
Hussain, Iftak [1 ,2 ]
Bowden, Audrey K. [1 ,2 ]
Haselton, Frederick R. [1 ,3 ]
机构
[1] Vanderbilt Univ, Dept Biomed Engn, Nashville, TN 37232 USA
[2] Vanderbilt Univ, Vanderbilt Biophoton Ctr, Nashville, TN 37232 USA
[3] Vanderbilt Univ, Dept Chem, Nashville, TN 37232 USA
关键词
LOW-COST; HE-4; CREATININE; PROTEIN; DIAGNOSIS; SENSOR; ASSAY;
D O I
10.1038/s41598-021-01544-4
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Ovarian cancer has a poor cure rate and rates of relapse are high. Current recurrence detection is limited by non-specific methods such as blood testing and ultrasound. Based on reports that human epididymis four (HE4) / creatinine (CRE) ratios found in urine are elevated in ovarian cancers, we have developed a paper-based device that combines lateral flow technology and cell phone analysis to quantitatively measure HE4/CRE. Surrogate samples were used to test the performance over clinically expected HE4/CRE ratios. For HE4/CRE ratios of 2 to 47, the percent error was found to be 16.0% on average whether measured by a flatbed scanner or cell phone. There was not a significant difference between the results from the cell phone or scanner. Based on published studies, error in this method was less than the difference required to detect recurrence. This promising new tool, with further development, could be used at home or in low-resource settings to provide timely detection of ovarian cancer recurrence.
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页数:11
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