Molecular cloning and characterization of two novel cellulase genes from the mollusc Ampullaria crossean

被引：42

作者：

Guo, Rui ^{[1
]}

Ding, Ming ^{[2
]}

Zhang, Si-Liang ^{[1
]}

Xu, Gen-Jun ^{[2
,3
]}

Zhao, Fu-Kun ^{[2
,3
]}

机构：

[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[2] Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Proteom, Inst Biochem & Cell Biol, Shanghai 20031, Peoples R China

[3] Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Peoples R China

来源：

JOURNAL OF COMPARATIVE PHYSIOLOGY B-BIOCHEMICAL SYSTEMS AND ENVIRONMENTAL PHYSIOLOGY | 2008年 / 178卷 / 02期

基金：

中国国家自然科学基金;

关键词：

cellulase; endoglucanase; EG27; Ampullaria crossean; gene cloning;

D O I：

10.1007/s00360-007-0214-z

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Cellulase genes have been reported not only from fungi, bacteria and plant, but also from some invertebrate animals. Here, two cellulase (endo-beta-1,4-glucanase, EC 3.2.1.4) genes, eg27I and eg27II, were cloned from the freshwater snail Ampullaria crossean cDNA using degenerate primers. The nucleotide sequences of the two genes shared 94.5% identity. The open reading frames of both genes consisted of 588 bp, encoding 195 amino acids. Both EG27I and EG27II belong to the glycoside hydrolase family 45, and each lacks a carbohydrate-binding module. The presence of introns demonstrated a eukaryotic origin of the EG27 gene, and, in addition, successful cloning of EG27 cDNA supported endogenous production of EG27 cellulase by Ampullaria crossean. Investigation of the EG27 cDNA from A. crossean will provide further information on GHF45 cellulases.

引用

页码：209 / 215

页数：7

共 24 条

[1] Structure and function of a cellulase gene in redclaw crayfish, Cherax quadricarinatus [J].

Crawford, AC ;

Kricker, JA ;

Anderson, AJ ;

Richardson, NR ;

Mather, PB .

GENE, 2004, 340 (02) :267-274

[2] Structures of oligosaccharide-bound forms of the endoglucanase V from Humicola insolens at 1.9 angstrom resolution [J].

Davies, GJ ;

Tolley, SP ;

Henrissat, B ;

Hjort, C ;

Schulein, M .

BIOCHEMISTRY, 1995, 34 (49) :16210-16220

[3] STRUCTURE AND FUNCTION OF ENDOGLUCANASE-V [J].

DAVIES, GJ ;

DODSON, GG ;

HUBBARD, RE ;

TOLLEY, SP ;

DAUTER, Z ;

WILSON, KS ;

HJORT, C ;

MIKKELSEN, JM ;

RASMUSSEN, G ;

SCHULEIN, M .

NATURE, 1993, 365 (6444) :362-364

[4] Ancient origin of glycosyl hydrolase family 9 cellulase genes [J].

Davison, A ;

Blaxter, M .

MOLECULAR BIOLOGY AND EVOLUTION, 2005, 22 (05) :1273-1284

[5]

Fan L. T., 1987, CELLULOSE HYDROLYSIS, DOI DOI 10.1007/978-3-642-72575-3_4

[6] DOMAINS IN MICROBIAL BETA-1,4-GLYCANASES - SEQUENCE CONSERVATION, FUNCTION, AND ENZYME FAMILIES [J].

GILKES, NR ;

HENRISSAT, B ;

KILBURN, DG ;

MILLER, RC ;

WARREN, RAJ .

MICROBIOLOGICAL REVIEWS, 1991, 55 (02) :303-315

[7] Molecular cloning of cDNAs encoding a range of digestive enzymes from a phytophagous beetle, Phaedon cochleariae [J].

Girard, C ;

Jouanin, L .

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1999, 29 (12) :1129-1142

[8] Molecular cloning and characterization of a cellulase gene from a symbiotic protist of the lower termite, Coptotermes, formosanus [J].

Inoue, T ;

Moriya, S ;

Ohkuma, M ;

Kudo, T .

GENE, 2005, 349 :67-75

[9] Purification and properties of a low molecular weight 1,4-β-D-glucan glucohydrolase having one active site for carboxymethyl cellulose and xylan from an alkalothermophilic Thermomonospora sp [J].

Jagtap, S ;

Rao, M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 329 (01) :111-116

[10] A family of glycosyl hydrolase family 45 cellulases from the pine wood nematode Bursaphelenchus xylophilus [J].

Kikuchi, T ;

Jones, JT ;

Aikawa, T ;

Kosaka, H ;

Ogura, N .

FEBS LETTERS, 2004, 572 (1-3) :201-205

← 1 2 3 →