BLV-CoCoMo-qPCR-2: improvements to the BLV-CoCoMo-qPCR assay for bovine leukemia virus by reducing primer degeneracy and constructing an optimal standard curve

被引：47
作者：
Takeshima, Shin-nosuke [1 ,2 ]
Kitamura-Muramatsu, Yuri [3 ]
Yuan, Yuan [3 ]
Polat, Meripet [1 ,2 ]
Saito, Susumu [3 ]
Aida, Yoko [1 ,2 ]
机构：
[1] RIKEN, Viral Infect Dis Unit, Wako, Saitama 3510198, Japan
[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Lab Viral Infect Dis, Wako, Saitama 3510198, Japan
[3] RIKEN GENESIS CO LTD, Riken Yokohama Inst, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan
来源：
ARCHIVES OF VIROLOGY | 2015年 / 160卷 / 05期
基金：
日本学术振兴会;
关键词：
Bovine leukemia virus; Quantitative PCR; BLV-CoCoMo-qPCR; Cattle; CoCoMo algorithm; Viral load; T-CELL LEUKEMIA; INFECTIOUS MOLECULAR CLONE; PROVIRAL LOAD; CATTLE; DESIGN; TOOL; DNA;
D O I：
10.1007/s00705-015-2377-3
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Because BLV infection can remain clinically silent, the proviral load is an important index for estimating disease progression. CoCoMo-qPCR, an assay developed to estimate BLV proviral load, allows the highly sensitive detection of BLV originating in different countries. Here, we developed a modified version of the CoCoMo-qPCR assay, the "BLV-CoCoMo-qPCR-2" assay, which uses optimized degenerate primers. We also constructed a new plasmid standard. Finally, we used both assays to examine DNA samples from BLV-infected cattle and compared the results.
引用
收藏
页码：1325 / 1332
页数：8
相关论文
共 19 条
[1]   PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].
ADACHI, A ;
GENDELMAN, HE ;
KOENIG, S ;
FOLKS, T ;
WILLEY, R ;
RABSON, A ;
MARTIN, MA .
JOURNAL OF VIROLOGY, 1986, 59 (02) :284-291
[2]   Mechanisms of pathogenesis induced by bovine leukemia virus as a model for human T-cell leukemia virus [J].
Aida, Yoko ;
Murakami, Hironobu ;
Takahashi, Masahiko ;
Takeshima, Shin-Nosuke .
FRONTIERS IN MICROBIOLOGY, 2013, 4
[3]   Bovine Leukemia Virus DNA in Human Breast Tissue [J].
Buehring, Gertrude Case ;
Shen, Hua Min ;
Jensen, Hanne M. ;
Choi, K. Yeon ;
Sun, Dejun ;
Nuovo, Gerard .
EMERGING INFECTIOUS DISEASES, 2014, 20 (05) :772-782
[4]   DegePrime, a Program for Degenerate Primer Design for Broad-Taxonomic-Range PCR in Microbial Ecology Studies [J].
Hugerth, Luisa W. ;
Wefer, Hugo A. ;
Lundin, Sverker ;
Jakobsson, Hedvig E. ;
Lindberg, Mathilda ;
Rodin, Sandra ;
Engstrand, Lars ;
Andersson, Anders F. .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (16) :5116-5123
[5]   Transmission and propagation in cell culture of virus produced by cells transfected with an infectious molecular clone of bovine leukemia virus [J].
Inabe, K ;
Ikuta, K ;
Aida, Y .
VIROLOGY, 1998, 245 (01) :53-64
[6]   BLV-CoCoMo-qPCR: a useful tool for evaluating bovine leukemia virus infection status [J].
Jimba, Mayuko ;
Takeshima, Shin-nosuke ;
Murakami, Hironobu ;
Kohara, Junko ;
Kobayashi, Naohiko ;
Matsuhashi, Tamako ;
Ohmori, Takashi ;
Nunoya, Tetsuo ;
Aida, Yoko .
BMC VETERINARY RESEARCH, 2012, 8
[7]   BLV-CoCoMo-qPCR: Quantitation of bovine leukemia virus proviral load using the CoCoMo algorithm [J].
Jimba, Mayuko ;
Takeshima, Shin-nosuke ;
Matoba, Kazuhiro ;
Endoh, Daiji ;
Aida, Yoko .
RETROVIROLOGY, 2010, 7
[8]   GENOMIC STRUCTURE OF HTLV (HUMAN T-CELL LEUKEMIA-VIRUS) - DETECTION OF DEFECTIVE GENOME AND ITS AMPLIFICATION IN MT-2 CELLS [J].
KOBAYASHI, N ;
KONISHI, H ;
SABE, H ;
SHIGESADA, K ;
NOMA, T ;
HONJO, T ;
HATANAKA, M .
EMBO JOURNAL, 1984, 3 (06) :1339-1343
[9]  
Konishi H, 1984, Mol Biol Med, V2, P273
[10]   POLYMERASE CHAIN-REACTION ANALYSIS OF DEFECTIVE HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I PROVIRAL GENOMES IN LEUKEMIC-CELLS OF PATIENTS WITH ADULT T-CELL LEUKEMIA [J].
KORBER, B ;
OKAYAMA, A ;
DONNELLY, R ;
TACHIBANA, N ;
ESSEX, M .
JOURNAL OF VIROLOGY, 1991, 65 (10) :5471-5476
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