BLV-CoCoMo-qPCR-2: improvements to the BLV-CoCoMo-qPCR assay for bovine leukemia virus by reducing primer degeneracy and constructing an optimal standard curve

被引：45

作者：

Takeshima, Shin-nosuke ^{[1
,2
]}

Kitamura-Muramatsu, Yuri ^{[3
]}

Yuan, Yuan ^{[3
]}

Polat, Meripet ^{[1
,2
]}

Saito, Susumu ^{[3
]}

Aida, Yoko ^{[1
,2
]}

机构：

[1] RIKEN, Viral Infect Dis Unit, Wako, Saitama 3510198, Japan

[2] Univ Tokyo, Grad Sch Frontier Sci, Dept Med Genome Sci, Lab Viral Infect Dis, Wako, Saitama 3510198, Japan

[3] RIKEN GENESIS CO LTD, Riken Yokohama Inst, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

来源：

ARCHIVES OF VIROLOGY | 2015年 / 160卷 / 05期

基金：

日本学术振兴会;

关键词：

Bovine leukemia virus; Quantitative PCR; BLV-CoCoMo-qPCR; Cattle; CoCoMo algorithm; Viral load; T-CELL LEUKEMIA; INFECTIOUS MOLECULAR CLONE; PROVIRAL LOAD; CATTLE; DESIGN; TOOL; DNA;

D O I：

10.1007/s00705-015-2377-3

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. Because BLV infection can remain clinically silent, the proviral load is an important index for estimating disease progression. CoCoMo-qPCR, an assay developed to estimate BLV proviral load, allows the highly sensitive detection of BLV originating in different countries. Here, we developed a modified version of the CoCoMo-qPCR assay, the "BLV-CoCoMo-qPCR-2" assay, which uses optimized degenerate primers. We also constructed a new plasmid standard. Finally, we used both assays to examine DNA samples from BLV-infected cattle and compared the results.

引用

页码：1325 / 1332

页数：8

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