Simultaneous Determination of Dextromethorphan and Quinidine Contents in Biological Fluid Samples Using Excitation-Emission Matrix Fluorescence Coupled with Second-Order Calibration Methods

This paper presents a novel method for simultaneous determination of dextromethorphan and quinidine contents in biological fluid samples using excitation-emission matrix fluorescence (EEM) coupled with second-order calibration methods. The adopted calibration methods, i.e., parallel factor analysis (PARAFAC), self-weighted alternating tri-linear decomposition (SWATLD), and alternating penalty trilinear decomposition (APTLD), could adequately exploit the second-order advantage. The accuracy of the three methods was evaluated through figures of merit and elliptical joint confidence region (EJCR) tests. It has been found that all the methods could give good results. But, with correct number of factors, the PARAFAC model performed slightly better than the others.