Production of polyclonal antibodies against peptide antigens using polystyrene beads as a carrier

被引：2
作者：
Kim, Mihee
Yun, Chul-Ho
Park, Seong-Ki
Seo, Jung-Hoon
Ahn, Taeho
机构：
[1] Chonnam Natl Univ, Coll Vet Med, Dept Biochem, Kwangju 500757, South Korea
[2] Chonnam Natl Univ, Dept Mol Endocrinol, Kwangju 500757, South Korea
[3] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea
来源：
BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 11期
关键词：
carrier; conjugation; peptide antigen; polyclonal antibody; polystyrene bead;
D O I：
10.1007/s10529-007-9463-x
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We describe a method for producing polyclonal antibody (PAb) against peptide antigen (the epitope region of cytochromes P450 1A2 and 3A4, HA tag, FLAG tag, and c-myc tag) using a polymer bead as a carrier. Carboxylated polystyrene beads (0.05, 0.5, 1, and 2 mu m diam) were conjugated with the peptide, which is known as an epitope region, using a chemical cross-linker and injected into rabbits with adjuvant. By immunizing the animals intradermally twice at two week intervals, an immunoblot assay with anti-sera showed the efficient generation of the PAb.
引用
收藏
页码：1735 / 1740
页数：6
相关论文
共 16 条
  • [1] Involvement of nonlamellar-prone lipids in the stability increase of human cytochrome P450 1A2 in reconstituted membranes
    Ahn, T
    Yun, CH
    Oh, DB
    [J]. BIOCHEMISTRY, 2005, 44 (25) : 9188 - 9196
  • [2] Angeletti R H, 1999, J Biomol Tech, V10, P2
  • [3] CONVENIENT MICROMETHOD FOR THE ASSAY OF PRIMARY AMINES AND PROTEINS WITH FLUORESCAMINE - RE-EXAMINATION OF THE CONDITIONS OF REACTION
    CASTELL, JV
    CERVERA, M
    MARCO, R
    [J]. ANALYTICAL BIOCHEMISTRY, 1979, 99 (02) : 379 - 391
  • [4] SHORT SYNTHETIC PEPTIDES EXPLOITED FOR RELIABLE AND SPECIFIC TARGETING OF ANTIBODIES TO THE C-TERMINI OF CYTOCHROME-P450 ENZYMES
    EDWARDS, RJ
    SINGLETON, AM
    MURRAY, BP
    DAVIES, DS
    BOOBIS, AR
    [J]. BIOCHEMICAL PHARMACOLOGY, 1995, 49 (01) : 39 - 47
  • [5] ISOLATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN C-MYC PROTO-ONCOGENE PRODUCT
    EVAN, GI
    LEWIS, GK
    RAMSAY, G
    BISHOP, JM
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) : 3610 - 3616
  • [6] PURIFICATION OF A RAS-RESPONSIVE ADENYLYL CYCLASE COMPLEX FROM SACCHAROMYCES-CEREVISIAE BY USE OF AN EPITOPE ADDITION METHOD
    FIELD, J
    NIKAWA, J
    BROEK, D
    MACDONALD, B
    RODGERS, L
    WILSON, IA
    LERNER, RA
    WIGLER, M
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (05) : 2159 - 2165
  • [7] Short peptide sequences containing MHC class I and/or class II epitopes linked to nano-beads induce strong immunity and inhibition of growth of antigen-specific tumour challenge in mice
    Fifis, T
    Mottram, P
    Bogdanoska, V
    Hanley, J
    Plebanski, M
    [J]. VACCINE, 2004, 23 (02) : 258 - 266
  • [8] HARLOW E, 1988, ANTIBODIES LAB MANUA, P128
  • [9] Membrane properties induced by anionic phospholipids and phosphatidylethanolamine are critical for the membrane binding and catalytic activity of human cytochrome p450 3A4
    Kim, KH
    Ahn, T
    Yun, CH
    [J]. BIOCHEMISTRY, 2003, 42 (51) : 15377 - 15387
  • [10] KNAPPIK A, 1994, BIOTECHNIQUES, V17, P754
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