Birth of cloned miniature pigs derived from somatic cell nuclear transferred embryos activated by ultrasound treatment

被引:38
作者
Miyoshi, Kazuchika
Inoue, Sumiko
Himaki, Takehiro
Mikawa, Satoshi
Yoshida, Mitsutoshi
机构
[1] Kagoshima Univ, Fac Agr, Lab Anim Reprod, Kagoshima 8900065, Japan
[2] Natl Inst Agrobiol Sci, Dept Anim Breeding & Genet, Tsukuba, Ibaraki, Japan
关键词
activation; nuclear transfer; ultrasound; in vivo development; miniature pig;
D O I
10.1002/mrd.20730
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The present study was carried out to determine (1) the optimal duty cycle of ultrasound for activation of pig oocytes and cloned embryos derived from miniature pig fetal fibroblasts and (2) whether cloned embryos can develop to term following activation by ultrasound stimulation. When oocytes were exposed to ultrasound with 20% or 30% duty cycle, the blastocyst formation rates were significantly (P < 0.05) higher than that of oocytes exposed to ultrasound with 10% duty cycle. In contrast, the blastocyst formation rate of cloned embryos decreased as the duty cycle of ultrasound increased; the value of embryos exposed to ultrasound with 10% duty cycle was significantly (P < 0.05) higher than that of embryos exposed to ultrasound with 50% duty cycle. When cloned embryos exposed to ultrasound with 10% duty cycle were transferred into the oviducts of two recipient gilts to assess their development in vivo, the pregnancy of one of the gilts was maintained to term and two piglets were delivered via Cesarean section. The results of the present study showed that (1) although the duty cycle of ultrasound affects in vitro development after activation of both pig oocytes and miniature pig cloned embryos, the optimal duty cycle is different between them and (2) miniature pig cloned embryos have the ability to develop into piglets after activation by ultrasound stimulation.
引用
收藏
页码:1568 / 1574
页数:7
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