Isolation, identification and functional profile of excretory-secretory peptides from Onchocerca ochengi

被引：13

作者：

Eberle, Raphael ^{[1
]}

Brattig, Norbert W. ^{[2
]}

Trusch, Maria ^{[3
]}

Schlueter, Hartmut ^{[4
]}

Achukwi, Mbunkah Daniel ^{[5
]}

Eisenbarth, Albert ^{[6
]}

Renz, Alfons ^{[6
]}

Liebau, Eva ^{[7
]}

Perbandt, Markus ^{[1
,8
]}

Betzel, Christian ^{[1
]}

机构：

[1] Univ Hamburg, Inst Biochem & Mol Biol, Lab Struct Biol Infect & Inflammat, DESY, D-22603 Hamburg, Germany

[2] Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany

[3] Univ Hamburg, Inst Organ Chem, D-20146 Hamburg, Germany

[4] Univ Med Ctr Hamburg Eppendorf UKE, Inst Clin Chem, D-20246 Hamburg, Germany

[5] Wakwa Ctr, Vet Res Lab, Inst Agr Res Dev, Ngaoundere, Cameroon

[6] Univ Tubingen, Inst Evolut & Ecol, D-72076 Tubingen, Germany

[7] Univ Munster, Inst Anim Physiol, D-48143 Munster, Germany

[8] Univ Med Ctr Hamburg Eppendorf UKE, Inst Med Microbiol Virol & Hyg, D-20246 Hamburg, Germany

来源：

ACTA TROPICA | 2015年 / 142卷

关键词：

Antibacterial activity; Excretory-secretory products; Filaria; Functional peptides; Onchocerca; HOST-DEFENSE PEPTIDES; ANTIMICROBIAL PEPTIDES; BRUGIA-MALAYI; HELMINTH-PARASITES; FILARIAL PARASITE; VOLVULUS; SERINE; EXPRESSION; PROTEINS;

D O I：

10.1016/j.actatropica.2014.11.015

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Parasitic helminths excrete or secrete a variety of functional molecules into the internal milieu of their mammalian hosts and arthropod vectors which reveal distinct immunomodulatory and other biological activities. We identified and initially characterized the low molecular weight peptide composition of the secretome from the filarial parasite Onchocerca ochengi. A total of 85 peptides were purified by liquid chromatography and further characterized by mass spectrometry. 72 of these peptides were derived from already described Onchocerca proteins and 13 peptide sequences are included in the sequence of uncharacterized proteins. Three peptides, similar to host defense peptides, revealed antibacterial activity. The present analysis confirms the putative involvement of low molecular weight compounds in the parasite-host cross-talk. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：156 / 166

页数：11

共 63 条

[1]

Aarbiou J, 2002, J LEUKOCYTE BIOL, V72, P167

[2] Onchocerca ochengi acquisition in zebu Gudali cattle exposed to natural transmission:: parasite population dynamics and IgG antibody subclass responses to Ov10/Ov11 recombinant antigens [J].

Achukwi, MD ;

Harnett, W ;

Bradley, J ;

Renz, A .

VETERINARY PARASITOLOGY, 2004, 122 (01) :35-49

[3]

Afacan NJ, 2012, CURR PHARM DESIGN, V18, P807, DOI 10.2174/138161212799277617

[4] UNDERSTANDING CHRONIC NEMATODE INFECTIONS - EVOLUTIONARY CONSIDERATIONS, CURRENT HYPOTHESES AND THE WAY FORWARD [J].

BEHNKE, JM ;

BARNARD, CJ ;

WAKELIN, D .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1992, 22 (07) :861-907

[5] Brugia malayi Excreted/Secreted Proteins at the Host/Parasite Interface: Stage- and Gender-Specific Proteomic Profiling [J].

Bennuru, Sasisekhar ;

Semnani, Roshanak ;

Meng, Zhaojing ;

Ribeiro, Jose M. C. ;

Veenstra, Timothy D. ;

Nutman, Thomas B. .

PLOS NEGLECTED TROPICAL DISEASES, 2009, 3 (04)

[6] Cost-effective expression and purification of antimicrobial and host defense peptides in Escherichia coli [J].

Bommarius, B. ;

Jenssen, H. ;

Elliott, M. ;

Kindrachuk, J. ;

Pasupuleti, Mukesh ;

Gieren, H. ;

Jaeger, K-E ;

Hancock, R. E. W. ;

Kalman, D. .

PEPTIDES, 2010, 31 (11) :1957-1965

[7] Identification and characterization of onchoastacin, an astacin-like metalloproteinase from the filaria Onchocerca volvulus [J].

Borchert, Nadine ;

Becker-Pauly, Christoph ;

Wagner, Antje ;

Fischer, Peter ;

Stoecker, Walter ;

Brattig, Norbert W. .

MICROBES AND INFECTION, 2007, 9 (04) :498-506

[8] Identification of the lipid mediator prostaglandin E2 in tissue immune cells of humans infected with the filaria Onchocerca volvulus [J].

Brattig, Norbert W. ;

Schwohl, Arline ;

Hoerauf, Achim ;

Buettner, Dietrich W. .

ACTA TROPICA, 2009, 112 (02) :231-235

[9] Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? [J].

Brogden, KA .

NATURE REVIEWS MICROBIOLOGY, 2005, 3 (03) :238-250

[10] Identification of defensin-1, defensin-2, and CAP37/azurocidin as T-cell chemoattractant proteins released from interleukin-8-stimulated neutrophils [J].

Chertov, O ;

Michiel, DF ;

Xu, LL ;

Wang, JM ;

Tani, K ;

Murphy, WJ ;

Longo, DL ;

Taub, DD ;

Oppenheim, JJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (06) :2935-2940

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