LncRNA LINC00944 Promotes Tumorigenesis but Suppresses Akt Phosphorylation in Renal Cell Carcinoma

被引:22
作者
Chen, Chiheng [1 ]
Zheng, Hanxiong [1 ]
机构
[1] Sun Yat Sen Univ, Affiliated Hosp 8, Dept Urol, Shenzhen, Peoples R China
关键词
LINC00944; tumorigenesis; Akt; phosphorylation; RCC; LONG NONCODING RNAS; CANCER STATISTICS; HOTAIR;
D O I
10.3389/fmolb.2021.697962
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Long non-coding RNA (lncRNA) is a kind of RNA that possesses longer than 200 nucleotides and lacks protein coding function. It was recognized as a junk sequence for a long time. Recent studies have found that lncRNAs are actively functioning in almost every aspect of cell biology and involved in a variety of biological functions. LncRNAs are closely related to a variety of human diseases, especially tumors. Recently, lncRNAs are being increasingly reported in renal cancer. In our study, we identified the expression of lncRNA LINC00944 is significantly elevated in renal cell carcinoma (RCC) tissues and cell lines and high LINC00944 expression is significantly correlated with the tumor stage and prognosis of RCC. The knockdown of LINC00944 by CRISPR/dCas9-KRAB in higher expressing 786-O and 769-P RCC cells could significantly decrease proliferation and migration and also promote phosphorylation of Akt compared with the control group. Our study is the first to report the function of lncRNA LINC00944 in RCC. And we provide clinicopathological and experimental evidence that lncRNA LINC00944 acts as an oncogene in RCC, suggesting that targeting lncRNA LINC00944 expression might be a promising therapeutic strategy for the treatment of RCC.
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页数:9
相关论文
共 24 条
[1]  
Bukowski RM, 2000, SEMIN ONCOL, V27, P204
[2]   Cancer Statistics in China, 2015 [J].
Chen, Wanqing ;
Zheng, Rongshou ;
Baade, Peter D. ;
Zhang, Siwei ;
Zeng, Hongmei ;
Bray, Freddie ;
Jemal, Ahmedin ;
Yu, Xue Qin ;
He, Jie .
CA-A CANCER JOURNAL FOR CLINICIANS, 2016, 66 (02) :115-132
[3]   Long Non-coding RNA HOTAIR Is Targeted and Regulated by miR-141 in Human Cancer Cells [J].
Chiyomaru, Takeshi ;
Fukuhara, Shinichiro ;
Saini, Sharanjot ;
Majid, Shahana ;
Deng, Guoren ;
Shahryari, Varahram ;
Chang, Inik ;
Tanaka, Yuichiro ;
Enokida, Hideki ;
Nakagawa, Masayuki ;
Dahiya, Rajvir ;
Yamamura, Soichiro .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2014, 289 (18) :12550-12565
[4]   Expression analysis and in silico characterization of intronic long noncoding RNAs in renal cell carcinoma: emerging functional associations [J].
Fachel, Angela A. ;
Tahira, Ana C. ;
Vilella-Arias, Santiago A. ;
Maracaja-Coutinho, Vinicius ;
Gimba, Etel R. P. ;
Vignal, Giselle M. ;
Campos, Franz S. ;
Reis, Eduardo M. ;
Verjovski-Almeida, Sergio .
MOLECULAR CANCER, 2013, 12
[5]   RNA in unexpected places: long non-coding RNA functions in diverse cellular contexts [J].
Geisler, Sarah ;
Coller, Jeff .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2013, 14 (11) :699-712
[6]   Epidemiologic and socioeconomic burden of metastatic renal cell carcinoma (mRCC): A literature review [J].
Gupta, Kiran ;
Miller, Jeffrey D. ;
Li, Jim Z. ;
Russel, Mason W. ;
Charbonneau, Claudie .
CANCER TREATMENT REVIEWS, 2008, 34 (03) :193-205
[7]   Long Noncoding RNA MALAT1 Promotes Aggressive Renal Cell Carcinoma through Ezh2 and Interacts with miR-205 [J].
Hirata, Hiroshi ;
Hinoda, Yuji ;
Shahryari, Varahram ;
Deng, Guoren ;
Nakajima, Koichi ;
Tabatabai, Z. Laura ;
Ishii, Nobuhisa ;
Dahiya, Rajvir .
CANCER RESEARCH, 2015, 75 (07) :1322-1331
[8]   How do lncRNAs regulate transcription? [J].
Long, Yicheng ;
Wang, Xueyin ;
Youmans, Daniel T. ;
Cech, Thomas R. .
SCIENCE ADVANCES, 2017, 3 (09)
[9]   2004 WHO classification of the renal tumors of the adults [J].
Lopez-Beltran, A ;
Scarpelli, M ;
Rodolfo, M ;
Kirkali, Z .
EUROPEAN UROLOGY, 2006, 49 (05) :798-805
[10]   Characterization of long non-coding RNA transcriptome in clear-cell renal cell carcinoma by next-generation deep sequencing [J].
Malouf, Gabriel G. ;
Zhang, Jianping ;
Yuan, Ying ;
Comperat, Eva ;
Roupret, Morgan ;
Cussenot, Olivier ;
Chen, Yunxin ;
Thompsong, Erika J. ;
Tannir, Nizar M. ;
Weinstein, John N. ;
Valero, Vicente ;
Khayat, David ;
Spano, Jean-Philippe ;
Su, Xiaoping .
MOLECULAR ONCOLOGY, 2015, 9 (01) :32-43