Rapid Detection of Mycoplasma bovis, Staphylococcus aureus and Streptococcus agalactiae in Cattle Bulk Tank Milk in Cyprus and Relations with Somatic Cell Counts

被引：7

作者：

Liapi, Maria ^{[1
,2
]}

Botsaris, George ^{[1
]}

Arsenoglou, Costas ^{[2
]}

Markantonis, Nikolas ^{[1
]}

Michael, Christodoulos ^{[1
]}

Antoniou, Antonis ^{[3
]}

Pipis, Christodoulos ^{[2
]}

机构：

[1] Cyprus Univ Technol, Dept Agr Sci Biotechnol & Food Sci, CY-3036 Limassol, Cyprus

[2] Cyprus Vet Serv, 1417 Athalassas Av, CY-1417 Nicosia, Cyprus

[3] Cyprus Inst Neurol & Genet, CY-1683 Nicosia, Cyprus

来源：

PATHOGENS | 2021年 / 10卷 / 07期

关键词：

Bovine mastitis; Mycoplasma bovis; Staphylococcus aureus; Streptococcus agalactiae; somatic cells; DAIRY HERDS; MASTITIS; PREVALENCE; IDENTIFICATION; MANAGEMENT; ASSAY;

D O I：

10.3390/pathogens10070841

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

One hundred and seventy-seven (177) bulk tank milk samples were analyzed with a commercially available real-time polymerase chain reaction kit and 11 (6.21%), 41 (23.16%), and 58 (32.77%) tested positive for Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae, respectively. Statistical analysis revealed a significant relationship between the presence of S. aureus and S. agalactiae. Enumeration of somatic cells was performed in the same samples by flow cytometry. The somatic cell counts were found higher in S. aureus and S. agalactiae positive samples. No association was found between M. bovis presence and somatic cells counts. Low internal assay control Ct values were found to be related with high somatic cell counts. Noticeably, this is the first report for the presence of M. bovis in Cyprus. Therefore, its presence was confirmed by bulk tank milk culture, conventional PCR, and next generation sequencing. Furthermore, M. bovis was typed with multilocus sequencing typing and was allocated to sequence type 29 (ST 29). Real-time PCR in bulk tank milk samples is a useful tool to detect mammary infections, especially for neglected pathogens such as M. bovis.

引用

页数：9

共 34 条

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