In vitro postovulatory oocyte aging affects H3K9 trimethylation in two-cell embryos after IVF

被引：10

作者：

Petri, Theresa ^{[1
]}

Dankert, Debora ^{[1
]}

Demond, Hannah ^{[2
,3
]}

Wennemuth, Gunther ^{[1
]}

Horsthemke, Bernhard ^{[2
]}

Gruemmer, Ruth ^{[1
]}

机构：

[1] Univ Duisburg Essen, Univ Hosp Essen, Inst Anat, Essen, Germany

[2] Univ Duisburg Essen, Univ Hosp Essen, Inst Human Genet, Essen, Germany

[3] Babraham Inst, Cambridge CB21 3AT, England

来源：

ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER | 2020年 / 227卷

关键词：

Postovulatory aging; Oocyte; Histone methylation; Embryonic development; INTRACYTOPLASMIC SPERM INJECTION; MOUSE OOCYTES; HISTONE MODIFICATIONS; EPIGENETIC MODIFICATIONS; CHROMATIN MODIFICATIONS; LYSINE-9; METHYLATION; DNA METHYLATION; LONG-TERM; FERTILIZATION; EXPRESSION;

D O I：

10.1016/j.aanat.2019.151424

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

Background: The physiological time axis of oocyte maturation comprises highly sensitive processes. A prolonged time span between ovulation and fertilization may impair oocyte developmental competence and subsequent embryo development, possibly due to epigenetic modifications. Since post-translational histone modifications can modify chromatin activity, and trimethylation of H3K9 (H3K9me3) has been shown to increase in the murine oocyte during maturation, here the effect of postovulatory oocyte aging on H3K9me3 was analyzed. Methods: The competence of murine oocytes which were aged for 2, 4, 6 and 8h in vitro after oocyte retrieval to develop to the two-cell and blastocyst stage was determined. Degree of H3K9me3 was analyzed in the postovulatory aged oocytes as well as in the resulting two-cell embryos after IVF. Results: The current study shows that postovulatory aging of oocytes for up to eight hours after oocyte retrieval exhibited no effect on two-cell embryo and blastocyst rate; however, changes in H3K9me3 in the resulting two-cell embryos were observed. Conclusion: Prolonged postovulatory oocyte aging leads to epigenetic modifications of H3K9. Such modifications may affect the developmental capacity of embryos at post-implantation developmental stages. (C) 2019 Elsevier GmbH. All rights reserved.

引用

页数：7

共 65 条

[11] Histone Demethylase Expression Enhances Human Somatic Cell Nuclear Transfer Efficiency and Promotes Derivation of Pluripotent Stem Cells [J].

Chung, Young Gie ;

Matoba, Shogo ;

Liu, Yuting ;

Eum, Jin Hee ;

Lu, Falong ;

Jiang, Wei ;

Lee, Jeoung Eun ;

Sepilian, Vicken ;

Cha, Kwang Yul ;

Lee, Dong Ryul ;

Zhang, Yi .

CELL STEM CELL, 2015, 17 (06) :758-766

[12] Broad histone H3K4me3 domains in mouse oocytes modulate maternal-to-zygotic transition [J].

Dahl, John Arne ;

Jung, Inkyung ;

Aanes, Havard ;

Greggains, Gareth D. ;

Manaf, Adeel ;

Lerdrup, Mads ;

Li, Guoqiang ;

Kuan, Samantha ;

Li, Bin ;

Lee, Ah Young ;

Preissl, Sebastian ;

Jermstad, Ingunn ;

Haugen, Mads Haugland ;

Suganthan, Rajikala ;

Bjoras, Magnar ;

Hansen, Klaus ;

Dalen, Knut Tomas ;

Fedorcsak, Peter ;

Ren, Bing ;

Klungland, Arne .

NATURE, 2016, 537 (7621) :548-+

[13]

Dankert D., 2014, NATURE, V537, P548

[14] Preovulatory Aging In Vivo and In Vitro Affects Maturation Rates, Abundance of Selected Proteins, Histone Methylation Pattern and Spindle Integrity in Murine Oocytes [J].

Demond, Hannah ;

Trapphoff, Tom ;

Dankert, Deborah ;

Heiligentag, Martyna ;

Gruemmer, Ruth ;

Horsthemke, Bernhard ;

Eichenlaub-Ritter, Ursula .

PLOS ONE, 2016, 11 (09)

[15] SPONTANEOUS ZONA REACTION IN THE MOUSE AS A LIMITING FACTOR FOR THE TIME IN WHICH AN OOCYTE MAY BE FERTILIZED [J].

DODSON, MG ;

MINHAS, BS ;

CURTIS, SK ;

PALMER, TV ;

ROBERTSON, JL .

JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER, 1989, 6 (02) :101-106

[16] DNA methylation pathways and their crosstalk with histone methylation [J].

Du, Jiamu ;

Johnson, Lianna M. ;

Jacobsen, Steven E. ;

Patel, Dinshaw J. .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2015, 16 (09) :519-532

[17] Dynamics of the epigenetic landscape during the maternal-to-zygotic transition [J].

Eckersley-Maslin, Melanie A. ;

Alda-Catalinas, Celia ;

Reik, Wolf .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2018, 19 (07) :436-450

[18] Mammalian oocyte development: checkpoints for competence [J].

Fair, Trudee .

REPRODUCTION FERTILITY AND DEVELOPMENT, 2010, 22 (01) :13-20

[19] Histone Modifications at the Blastocyst Axin1Fu Locus Mark the Heritability of In Vitro Culture-Induced Epigenetic Alterations in Mice [J].

Fernandez-Gonzalez, Raul ;

Ramirez, Miguel A. ;

Pericuesta, Eva ;

Calle, Alexandra ;

Gutierrez-Adan, Alfonso .

BIOLOGY OF REPRODUCTION, 2010, 83 (05) :720-727

[20] Regulation of H3K27me3 and H3K4me3 During Early Porcine Embryonic Development [J].

Gao, Yu ;

Hyttel, Poul ;

Hall, Vanessa Jane .

MOLECULAR REPRODUCTION AND DEVELOPMENT, 2010, 77 (06) :540-549

← 1 2 3 4 5 6 7 →