Effects of fructooligosaccharides (FOS) on the composition of cecal and fecal microbiota and the quantitative detection of FOS-metabolizing bacteria using species-specific primers

被引：8

作者：

Gu, Jiayu ^{[1
,2
]}

Cui, Shumao ^{[1
,2
]}

Tang, Xin ^{[1
,2
]}

Liu, Zhenmin ^{[3
]}

Zhao, Jianxin ^{[1
,2
]}

Zhang, Hao ^{[1
,2
,4
]}

Mao, Bingyong ^{[1
,2
]}

Chen, Wei ^{[1
,2
,4
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi, Jiangsu, Peoples R China

[2] Jiangnan Univ, Sch Food Sci & Technol, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

[3] Bright Dairy & Food Co Ltd, Dairy Res Inst, Shanghai Engn Res Ctr Dairy Biotechnol, State Key Lab Dairy Biotechnol, Shanghai, Peoples R China

[4] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi, Jiangsu, Peoples R China

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 2022年 / 102卷 / 12期

基金：

中国国家自然科学基金;

关键词：

fructooligosaccharides; Bifidobacterium pseudolongum; 16S rDNA sequencing; FOS-metabolizing bacteria; species-specific quantification; CHAIN FATTY-ACIDS; FRUCTO-OLIGOSACCHARIDES; INTESTINAL MICROBIOTA; METAGENOMIC INSIGHTS; MICE; BIFIDOBACTERIA; FERMENTATION; MICROFLORA; FLORA;

D O I：

10.1002/jsfa.11884

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

BACKGROUND Fructooligosaccharides (FOS) are a kind of prebiotic. Previous studies concerning the effect of FOS on intestinal microbiota have focused on Bifidobacterium and Lactobacillus. However, the presence of other FOS-utilizing bacteria makes it necessary to investigate the quantitative changes in these bacterial species in the intestine after FOS intake. In this study, the composition of cecal and fecal microbiota was analyzed using MiSeq sequencing, and the abundance of FOS-utilizing bacteria was detected using quantitative polymerase chain reaction after the oral administration of FOS. RESULTS Species-specific primers for FOS-utilizing bacteria were designed with superior amplification efficiency for quantification. After FOS intervention, the relative abundance of Bifidobacterium pseudolongum in feces increased to 17.37% and the abundance reached 2.28 x 10(10) CFU g(-1). The abundance of Bifidobacterium longum and Bifidobacterium breve did not change significantly. Whereas the abundance of Ligilactobacillus murinus decreased, that of Lactiplantibacillus plantarum, Lacticaseibacillus paracasei, and Lacticaseibacillus rhamnosus remained at approximately 10(4) CFU g(-1). CONCLUSION Species-specific primers for FOS-utilizing bacteria were successfully developed, and we confirmed that FOS significantly increased the relative abundance and the abundance of B. pseudolongum in mice, while decreasing the proportion of Lactobacillus. The detection of these species using 16S ribosomal DNA sequencing and quantitative polymerase chain reaction showed the same results. Further investigations are needed to reveal the response of the intestinal microbiota to different FOS compositions. These techniques will contribute to future studies about the composition and dynamics of the intestinal microflora. (c) 2022 Society of Chemical Industry.

引用

页码：5301 / 5311

页数：11