Advanced microscopy to elucidate cardiovascular injury and regeneration: 4D light-sheet imaging

被引：22

作者：

Baek, Kyung In ^{[1
]}

Ding, Yichen ^{[2
]}

Chang, Chih-Chiang ^{[1
]}

Chang, Megan ^{[2
]}

Packard, Rene R. Sevag ^{[2
]}

Hsu, Jeffrey J. ^{[2
]}

Fei, Peng ^{[3
]}

Hsiai, Tzung K. ^{[1
,2
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Bioengn, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med, Los Angeles, CA 90095 USA

[3] Huazhong Univ Sci & Technol, Sch Opt & Elect Informat, Wuhan 430074, Hubei, Peoples R China

[4] CALTECH, Med Engn, Pasadena, CA 91106 USA

来源：

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY | 2018年 / 138卷

基金：

美国国家卫生研究院;

关键词：

Light-sheet fluorescence microscopy; Cardiovascular injury; Doxorubicin; Vascular regeneration; PLANE ILLUMINATION MICROSCOPY; ENDOTHELIAL-CELL METABOLISM; ULTRAFINE PARTICLE EXPOSURE; AMBIENT PARTICULATE MATTER; SHEAR-STRESS; AIR-POLLUTION; FLUORESCENCE MICROSCOPY; DISTURBED FLOW; PKC-EPSILON; HEART;

D O I：

10.1016/j.pbiomolbio.2018.05.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The advent of 4-dimensional (4D) light-sheet fluorescence microscopy (LSFM) has provided an entry point for rapid image acquisition to uncover real-time cardiovascular structure and function with high axial resolution and minimal photo-bleaching/-toxicity. We hereby review the fundamental principles of our LSFM system to investigate cardiovascular morphogenesis and regeneration after injury. LSFM enables us to reveal the micro-circulation of blood cells in the zebrafish embryo and assess cardiac ventricular remodeling in response to chemotherapy-induced injury using an automated segmentation approach. Next, we review two distinct mechanisms underlying zebrafish vascular regeneration following tail amputation. We elucidate the role of endothelial Notch signaling to restore vascular regeneration after exposure to the redox active ultrafine particles (UFP) in air pollutants. By manipulating the blood viscosity and subsequently, endothelial wall shear stress, we demonstrate the mechanism whereby hemodynamic shear forces impart both mechanical and metabolic effects to modulate vascular regeneration. Overall, the implementation of 4D LSFM allows for the elucidation of mechanisms governing cardiovascular injury and regeneration with high spatiotemporal resolution. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：105 / 115

页数：11

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