LGR4 Is Required for the Cell Survival of the Peripheral Mesenchyme at the Embryonic Stages of Nephrogenesis

被引：11
|
作者：
Mohri, Yasuaki [1 ]
Oyama, Kazunori [1 ]
Sone, Mizuki [1 ]
Akamatsu, Atsushi [1 ]
Nishimori, Katsuhiko [1 ]
机构：
[1] Tohoku Univ, Grad Sch Agr Sci, Mol Biol Lab, Aoba Ku, Sendai, Miyagi 9818555, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 05期
基金：
日本学术振兴会;
关键词：
LGR4; knockout mice; kidney morphogenesis; apoptosis; COUPLED RECEPTOR 48; MALE REPRODUCTIVE-TRACT; BRANCHING MORPHOGENESIS; METANEPHRIC MESENCHYME; POSTNATAL-DEVELOPMENT; REDUCED EXPRESSION; KIDNEY DEVELOPMENT; DOWN-REGULATION; MICE; PROTEIN;
D O I：
10.1271/bbb.110834
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In mice, homozygous Lgr4 inactivation results in hypoplastic kidneys. To understand better the role of LGR4 in kidney development, we performed an analysis of kidneys in Lgr4(-/-) embryos. We stained Lgr4(-/-) kidneys with anti-WT1 and anti-Cleaved Caspase3 antibodies at E16.5, and observed that the structures of the cap mesenchyme were disrupted and that apoptosis increased. In addition, the expression of PAX2, an anti-apoptotic factor in kidney development, was also significantly decreased at E16.5. We found that the LGR4 defect caused an increase in apoptosis in the peripheral mesenchyme during kidney development.
引用
收藏
页码：888 / 891
页数：4
相关论文
共 50 条
  • [31] Loss of the stress sensor GADD45A promotes stem cell activity and ferroptosis resistance in LGR4/HOXA9-dependent AML
    Hassan, Nunki
    Yi, Hangyu
    Malik, Bilal
    Gaspard-Boulinc, Lucie
    Samaraweera, Saumya E.
    Casolari, Debora A.
    Seneviratne, Janith
    Balachandran, Anushree
    Chew, Tracy
    Duly, Alastair
    Carter, Daniel R.
    Cheung, Belamy B.
    Norris, Murray
    Haber, Michelle
    Kavallaris, Maria
    Marshall, Glenn M.
    Zhang, Xu Dong
    Liu, Tao
    Wang, Jianlong
    Liebermann, Dan A.
    'Andrea, Richard J. D.
    Wang, Jenny Y.
    BLOOD, 2024, 144 (01) : 84 - 98
  • [32] Oct4 is required for primordial germ cell survival
    Kehler, J
    Tolkunova, E
    Koschorz, B
    Pesce, M
    Gentile, L
    Boiani, M
    Lomelí, H
    Nagy, A
    McLaughlin, KJ
    Schöler, HR
    Tomilin, A
    EMBO REPORTS, 2004, 5 (11) : 1078 - 1083
  • [33] Targeted Deletion of the Murine Lgr4 Gene Decreases Lens Epithelial Cell Resistance to Oxidative Stress and Induces Age-Related Cataract Formation
    Zhu, Jun
    Hou, Qiang
    Dong, Xiang Da
    Wang, Zhenlian
    Chen, Xiaoyan
    Zheng, Dandan
    Zhou, Linglin
    He, Chao
    Liu, Mingyao
    Tu, LiLi
    Qu, Jia
    PLOS ONE, 2015, 10 (03):
  • [34] beta 4 integrin is required for hemidesmosome formation, cell adhesion and cell survival
    Dowling, J
    Yu, QC
    Fuchs, E
    JOURNAL OF CELL BIOLOGY, 1996, 134 (02): : 559 - 572
  • [35] Integrin receptors are required for cell survival and proliferation during development of the peripheral glial lineage
    Haack, H
    Hynes, RO
    DEVELOPMENTAL BIOLOGY, 2001, 233 (01) : 38 - 55
  • [36] lan4 is required for mitochondrial integrity and T cell survival
    Pandarpurkar, M
    Wilson-Fritch, L
    Corvera, S
    Markholst, H
    Hornum, L
    Greiner, DL
    Mordes, JP
    Rossini, AA
    Bortell, R
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (18) : 10382 - 10387
  • [37] Integrin α-4β1 function is required for cell survival in developing retina
    Leu, ST
    Jacques, SAL
    Wingerd, KL
    Hikita, ST
    Tolhurst, EC
    Pring, JL
    Wiswell, D
    Kinney, L
    Goodman, NL
    Jackson, DY
    Clegg, DO
    DEVELOPMENTAL BIOLOGY, 2004, 276 (02) : 416 - 430
  • [38] Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice
    Ye, Buqing
    Liu, Benyu
    Hao, Lu
    Zhu, Xiaoxiao
    Yang, Liuliu
    Wang, Shuo
    Xia, Pengyan
    Du, Ying
    Meng, Shu
    Huang, Guanling
    Qin, Xiwen
    Wang, Yanying
    Yan, Xinlong
    Li, Chong
    Hao, Junfeng
    Zhu, Pingping
    He, Luyun
    Tian, Yong
    Fan, Zusen
    NATURE COMMUNICATIONS, 2018, 9
  • [39] Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice
    Buqing Ye
    Benyu Liu
    Lu Hao
    Xiaoxiao Zhu
    Liuliu Yang
    Shuo Wang
    Pengyan Xia
    Ying Du
    Shu Meng
    Guanling Huang
    Xiwen Qin
    Yanying Wang
    Xinlong Yan
    Chong Li
    Junfeng Hao
    Pingping Zhu
    Luyun He
    Yong Tian
    Zusen Fan
    Nature Communications, 9
  • [40] Ets1 and Ets2 are required for endothelial cell survival during embryonic angiogenesis
    Wei, Guo
    Srinivasan, Ruchika
    Cantemir-Stone, Carmen Z.
    Sharma, Sudarshana M.
    Santhanam, Ramasamy
    Weinstein, Michael
    Muthusamy, Natarajan
    Man, Albert K.
    Oshima, Robert G.
    Leone, Gustavo
    Ostrowski, Michael C.
    BLOOD, 2009, 114 (05) : 1123 - 1130
12345