LGR4 Is Required for the Cell Survival of the Peripheral Mesenchyme at the Embryonic Stages of Nephrogenesis

被引：11
|
作者：
Mohri, Yasuaki [1 ]
Oyama, Kazunori [1 ]
Sone, Mizuki [1 ]
Akamatsu, Atsushi [1 ]
Nishimori, Katsuhiko [1 ]
机构：
[1] Tohoku Univ, Grad Sch Agr Sci, Mol Biol Lab, Aoba Ku, Sendai, Miyagi 9818555, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2012年 / 76卷 / 05期
基金：
日本学术振兴会;
关键词：
LGR4; knockout mice; kidney morphogenesis; apoptosis; COUPLED RECEPTOR 48; MALE REPRODUCTIVE-TRACT; BRANCHING MORPHOGENESIS; METANEPHRIC MESENCHYME; POSTNATAL-DEVELOPMENT; REDUCED EXPRESSION; KIDNEY DEVELOPMENT; DOWN-REGULATION; MICE; PROTEIN;
D O I：
10.1271/bbb.110834
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In mice, homozygous Lgr4 inactivation results in hypoplastic kidneys. To understand better the role of LGR4 in kidney development, we performed an analysis of kidneys in Lgr4(-/-) embryos. We stained Lgr4(-/-) kidneys with anti-WT1 and anti-Cleaved Caspase3 antibodies at E16.5, and observed that the structures of the cap mesenchyme were disrupted and that apoptosis increased. In addition, the expression of PAX2, an anti-apoptotic factor in kidney development, was also significantly decreased at E16.5. We found that the LGR4 defect caused an increase in apoptosis in the peripheral mesenchyme during kidney development.
引用
收藏
页码：888 / 891
页数：4
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