Effects of Zibu Piyin Recipe on SNK-SPAR pathway in neuron injury induced by glutamate

被引:12
作者
Zhan Li-bin [1 ]
Sui Hua [2 ]
Lu Xiao-guang [3 ]
Sun Chang-kai [4 ]
Zhang Jian [4 ]
Ma Hui [4 ]
机构
[1] Dalian Med Univ, Affiliated Hosp 2, Dalian 116023, Peoples R China
[2] Dalian Med Univ, Dept Pathophysiol, Dalian 116044, Peoples R China
[3] Dalian Univ Technol, Zhongshan Hosp, Dept Emergency Med, Dalian 116001, Peoples R China
[4] Dalian Med Univ, Inst Brain Disorders, Dept Physiol, Dalian 116044, Peoples R China
基金
中国国家自然科学基金;
关键词
Alzheimer's disease; Zibu Piyin Recipe; serum-inducible kinase; spine-associated Rap GTPase-activating protein; excitotoxicity; dendritic spine;
D O I
10.1007/s11655-008-0117-1
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Objective: To investigate the relationship between the excitotoxicity and serum-inducible kinase (SNK) and spine-associated Rap GTPase-activating protein (SPAR) pathway in primary hippocampal neuron injury induced by glutamate and furthermore, to explore the molecular mechanism of neuroprotection of Zibu Piyin Recipe (ZBPYR) and the relationship between ZBPYR and the morphological regulation of dendritic spines. Methods: The serum containing ZBPYR was prepared by seropharmacology. Reverse transcription and polymerase chain reaction (RT-PCR) was used to detect the expression of mRNA for SNK, SPAR, postsynaptic density protein 95 (PSD-95) and N-methyl-D-aspartate (NMDA) receptor subunits (NR1, NR2A and NR2B) in primary rat hippocampal neuron cultures after pretreatment with 10 mu mol/L glutamate and ZBPYR serum. Results: ZBPYR serum pretreatment resulted in a significant down-regulation of glutamate-induced SNK mRNA expression (P<0.05). Significant up-regulation was seen on the mRNA expression of SPAR and PSD-95 (P<0.05). All these changes were dose-dependent. The mRNA expression of NR1, NR2A and NR213 was down-regulated to different degrees (P<0.05). Conclusion: The mechanism of effect of ZBPYR on glutamate-induced excitotoxicity may be related to the regulation of SNK-SPAR signal pathway. ZBPYR may play a role in protecting and maintaining the normal morphology and structure of dendritic spines, which may be achieved by inhibiting the excessive activation of NMDA receptors.
引用
收藏
页码:117 / 122
页数:6
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