Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy

被引：32

作者：

Lin, Po-Yen ^{[1
,2
]}

Lyu, Hong-Chou ^{[1
]}

Hsu, Chin-Ying Stephen ^{[3
]}

Chang, Chia-Seng ^{[2
]}

Kao, Fu-Jen ^{[1
,4
]}

机构：

[1] Natl Yang Ming Univ, Inst Biophoton, Taipei 11221, Taiwan

[2] Acad Sinica, Inst Phys, Taipei 11529, Taiwan

[3] Natl Univ Singapore, Natl Univ Hlth Syst, Dept Prevent Dent, Fac Dent, Singapore 119074, Singapore

[4] Natl Sun Yat Sen Univ, Dept Photon, Kaohsiung 80424, Taiwan

来源：

BIOMEDICAL OPTICS EXPRESS | 2011年 / 2卷 / 01期

关键词：

2ND-HARMONIC GENERATION; IN-VIVO; TIME; SPECTROSCOPY; AUTOFLUORESCENCE; CARIES; CELLS;

D O I：

10.1364/BOE.2.000149

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, multiphoton excitation was utilized to image normal and carious dental tissues noninvasively. Unique structures in dental tissues were identified using the available multimodality (second harmonic, autofluorescence, and fluorescence lifetime analysis) without labeling. The collagen in dentin exhibits a strong second harmonic response. Both dentin and enamel emit strong autofluorescence that reveals in detail morphological features (such as dentinal tubules and enamel rods) and, despite their very similar spectral profiles, can be differentiated by lifetime analysis. Specifically, the carious dental tissue exhibits a greatly reduced autofluorescence lifetime, which result is consistent with the degree of demineralization, determined by micro-computed tomography. Our findings suggest that two-photon excited fluorescence lifetime imaging may be a promising tool for diagnosing and monitoring dental caries. (C)2010 Optical Society of America

引用

页码：149 / 158

页数：10

共 36 条

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