Proopiomelanocortin (POMC) mRNA and POMC-derived peptides immunolocalization in the skin of Protopterus annectens, an African lungfish

被引：4

作者：

Masini, MA

Sturla, M

Pestarino, M

Facchinetti, F

Gallinelli, A

Uva, BM

机构：

[1] Univ Genoa, Dept Expt Environm & Appl Biol, I-16132 Genoa, Italy

[2] Univ Modena, Dept Obstet & Gynecol, I-41100 Modena, Italy

来源：

PEPTIDES | 1999年 / 20卷 / 01期

关键词：

POMC-related peptides; POMC mRNA; immunohistochemistry; skin; lungfish; Protopterus annectens; dipnoans;

D O I：

10.1016/S0196-9781(98)00145-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Antisera against adrenocorticotropic hormone (ACTH), alpha-melanocyte stimulating hormone (alpha-MSH) and beta-endorphin were used to localize, by immunohistochemistry, proopiomelanocortin (POMC)-derived peptides in the skin excised from different regions of the African lungfish Protopterus annectens. Immunoreactivity was observed in the epidermis mainly in the germinal layer. Using human POMC cDNA as hybridization probe, POMC-like mRNA was identified in situ in epidermal cells. The demonstration in the same cells of POMC mRNA and POMC-related peptides immunoreactivity indicates a local production of opiate hormones. (C) 1999 by Elsevier Science Inc.

引用

页码：87 / 91

页数：5

共 18 条

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