Hydrolytic fate of deoxynivalenol-3-glucoside during digestion

被引:214
作者
Berthiller, Franz [1 ]
Krska, Rudolf
Domig, Konrad J. [2 ]
Kneifel, Wolfgang [3 ]
Juge, Nathalie [4 ]
Schuhmacher, Rainer
Adam, Gerhard [5 ]
机构
[1] Univ Nat Resources & Life Sci, Christian Doppler Lab Mycotoxin Metab, Ctr Analyt Chem, Dept Agrobiotechnol IFA Tulln,Vienna BOKU, A-3430 Tulln, Austria
[2] Univ Nat Resources & Life Sci, Inst Food Sci, Dept Food Sci & Technol, Vienna BOKU, A-1190 Vienna, Austria
[3] Univ Nat Resources & Life Sci, Christian Doppler Lab Innovat Bran Biorefinery, Dept Food Sci & Technol, Vienna BOKU, A-1190 Vienna, Austria
[4] Inst Food Res, Norwich NR4 7UA, Norfolk, England
[5] Univ Nat Resources & Life Sci, Dept Appl Genet & Cell Biol, Vienna BOKU, A-3430 Tulln, Austria
基金
奥地利科学基金会;
关键词
Deoxynivalenol-3-glucoside; Deoxynivalenol; Conjugated mycotoxins; Masked mycotoxins; Digestion; Hydrolysis; HEAD BLIGHT RESISTANCE; MYCOTOXIN DEOXYNIVALENOL; UDP-GLUCOSYLTRANSFERASE; MASKED DEOXYNIVALENOL; WHEAT; EXPRESSION; GLUCOSIDE; BARLEY;
D O I
10.1016/j.toxlet.2011.08.006
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Deoxynivalenol-3-beta-D-glucoside (D3G), a plant phase II metabolite of the Fusarium mycotoxin deoxynivalenol (DON), occurs in naturally contaminated wheat, maize, oat, barley and products thereof. Although considered as a detoxification product in plants, the toxicity of this substance in mammals is currently unknown. A major concern is the possible hydrolysis of the D3G conjugate back to its toxic precursor mycotoxin DON during mammalian digestion. We used in vitro model systems to investigate the stability of D3G to acidic conditions, hydrolytic enzymes and intestinal bacteria, mimicking different stages of digestion. D3G was found resistant to 0.2 M hydrochloric acid for at least 24 h at 37 degrees C, suggesting that it will not be hydrolyzed in the stomach of mammals. While human cytosolic beta-glucosidase also had no effect, fungal cellulase and cellobiase preparations could cleave a significant portion of D3G. Most importantly, several lactic acid bacteria such as Enterococcus durans, Enterococcus mundtii or Lactobacillus plantarum showed a high capability to hydrolyze D3G. Taken together these data indicate that D3G is of toxicological relevance and should be regarded as a masked mycotoxin. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:264 / 267
页数:4
相关论文
共 27 条
[1]   Microbiology: Gut reaction [J].
Abbott, A .
NATURE, 2004, 427 (6972) :284-286
[2]  
[Anonymous], OFFICIAL J EUROPEAN
[3]   Functional expression of human liver cytosolic β-glucosidase in Pichia pastoris -: Insights into its role in the metabolism of dietary glucosides [J].
Berrin, JG ;
McLauchlan, WR ;
Needs, P ;
Williamson, G ;
Puigserver, A ;
Kroon, PA ;
Juge, N .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2002, 269 (01) :249-258
[4]   Masked mycotoxins: Determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrometry [J].
Berthiller, F ;
Dall'Asta, C ;
Schuhmacher, R ;
Lemmens, M ;
Adam, G ;
Krska, R .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (09) :3421-3425
[5]   Occurrence of deoxynivalenol and its 3--D-glucoside in wheat and maize [J].
Berthiller, F. ;
Dall'asta, C. ;
Corradini, R. ;
Marchelli, R. ;
Sulyok, M. ;
Krska, R. ;
Adam, G. ;
Schuhmacher, R. .
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2009, 26 (04) :507-511
[6]   Formation, determination and significance of masked and other conjugated mycotoxins [J].
Berthiller, Franz ;
Schuhmacher, Rainer ;
Adam, Gerhard ;
Krska, Rudolf .
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2009, 395 (05) :1243-1252
[7]   Glycosyltransferases of lipophilic small molecules [J].
Bowles, Dianna ;
Lim, Eng-Kiat ;
Poppenberger, Brigitte ;
Vaistij, Fabian E. .
ANNUAL REVIEW OF PLANT BIOLOGY, 2006, 57 :567-597
[8]   QTL mapping and marker-assisted selection for Fusarium head blight resistance in wheat: a review [J].
Buerstmayr, H. ;
Ban, T. ;
Anderson, J. A. .
PLANT BREEDING, 2009, 128 (01) :1-26
[9]   Survey of deoxynivalenol and deoxynivalenol-3-glucoside in cereal-based products by liquid chromatography electrospray ionization tandem mass spectrometry [J].
Desmarchelier, A. ;
Seefelder, W. .
WORLD MYCOTOXIN JOURNAL, 2011, 4 (01) :29-35
[10]  
Engelhardt Gabriele, 1999, Advances in Food Sciences, V21, P71