Quantitative Protease Cleavage Site Profiling using Tandem-Mass-Tag Labeling and LC-MALDI-TOF/TOF MS/MS Analysis

被引:20
|
作者
Jakoby, Thomas [1 ]
van den Berg, Bart H. J. [1 ]
Tholey, Andreas [1 ]
机构
[1] Univ Kiel, Inst Expt Med, AG Systemat Proteomforsch, D-24098 Kiel, Germany
关键词
relative quantification; PICS approach; peptide library; TMT; isobaric labeling; mass spectrometry; ACTIVITY-BASED PROBES; PEPTIDE LIBRARIES; PROTEOMIC IDENTIFICATION; STAPHYLOCOCCUS-AUREUS; ACTIVE-SITE; SPECTROMETRY; SPECIFICITY; DISCOVERY; CANCER; METALLOPROTEINASES;
D O I
10.1021/pr201051e
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Knowledge of cleavage site specificity and activity are major prerequisites for understanding protease function. On the basis of a recently presented approach for proteomic identification of cleavage sites (PICS) in proteome-derived peptide libraries, we developed an isobaric labeling quantitative LC-MALDI-TOF/TOF MS/MS approach (Q-PICS) for simultaneous determination of cleavage site specificity and robust relative quantification of proteolytic events. For GluC-protease, 737 cleavage sites were identified in a yeast proteome-derived peptide library; 94.0% showed the typical GluC specificity for peptide bonds at glutamyl and aspartyl residues. The six-plex tandem mass tagging strategy allowed for three simultaneous replicates in a single run, guaranteeing high confidence and robust statistics for quantitative measurements. Using the quantitative capacity of Q-PICS, we performed a comparison of cleavage site specificity of GluC in two different buffer systems. The results support earlier findings describing that apparent difference between the buffer systems are probably caused by the inhibitory effect of bicarbonate on the overall GluC activity and that the preference for Glu-X bonds compared to Asp-X bonds is independent of the buffer system used.
引用
收藏
页码:1812 / 1820
页数:9
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