Modulation of migratory activity and invasiveness of human glioma spheroids following 5-aminolevulinic acid based photodynamic treatment Laboratory investigation
Object. Five-aminolevulinic acid mediated photodynamic therapy (ALA/PDT) can improve the clinical outcome in patients suffering from glioblastoma. Besides direct phototoxicity, additional mechanisms may contribute. Therefore, the authors studied the influence of ALA/PDT on glioblastoma's migratory and invasive behavior in a human glioma cell spheroid model. Methods. Glioma spheroids were grown from human U373 and A172 cell lines. After ALA/PDT of spheroids, the authors assessed the migration of tumor cells and their capacity to invade a collagen matrix, as well as changes in their viability, morphology, and expression of matrix metalloproteinases (MMPs). Results. The authors found that ALA/PDT caused long-lasting, nearly complete suppression of glioma cell migration and matrix invasion compared with nontherapeutic controls, including either irradiation or incubation with ALA only. Although ALA/PDT induced tumor cell apoptosis, suppression of migration/invasion was not simply due to phototoxicity because 50% of tumor cells remained vital throughout the observation period. Moreover, the morphology of ALA/PDT-treated cells changed significantly toward a polygonal, epithelial-like appearance, which was associated with alterations in the actin cytoskeleton. Furthermore, downregulation of MMP-7 and -8 was observed after treatment whereas other MMPs remained unchanged. Conclusions. In addition to directly eliminating glioma cells through apoptosis. ALA/PDT alters their invasiveness, possibly due to the effects on the cytoskeletal organization and MMP expression. (DOI: 10.3171/2011.3.JNS10434)
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Acad Nacl Med Buenos Aires, Inst Estudios Oncol, RA-3092 Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Vanzulli, Silvia
Perotti, Christian
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Univ Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Univ Buenos Aires, Hosp Clin Jose de San Martin, Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Perotti, Christian
Mamome, Leandro
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Univ Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Univ Buenos Aires, Hosp Clin Jose de San Martin, Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Mamome, Leandro
Rodriguez, Lorena
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Univ Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Univ Buenos Aires, Hosp Clin Jose de San Martin, Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Rodriguez, Lorena
Simian, Marina
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h-index: 0
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Inst Angel H Roffo, RA-5481 Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Simian, Marina
Juarranz, Angeles
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h-index: 0
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Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, SpainUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Juarranz, Angeles
Pontiggia, Osvaldo
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h-index: 0
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Inst Angel H Roffo, RA-5481 Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Pontiggia, Osvaldo
Hasan, Tayyaba
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h-index: 0
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Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Dermatol,Wellman Labs Photomed WEL 224, Boston, MA 02114 USAUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Hasan, Tayyaba
Batlle, Alcira
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h-index: 0
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Univ Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Univ Buenos Aires, Hosp Clin Jose de San Martin, Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
机构:
Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Sanz-Rodriguez, Francisco
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Di Venosa, Gabriela
Rodriguez, Lorena
论文数: 0引用数: 0
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Univ Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Univ Buenos Aires, Hosp Clin Jose San Martin, Buenos Aires, ArgentinaUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Rodriguez, Lorena
Mamone, Leandro
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Univ Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Univ Buenos Aires, Hosp Clin Jose San Martin, Buenos Aires, ArgentinaUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Mamone, Leandro
Blazquez, Alfonso
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h-index: 0
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Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Blazquez, Alfonso
Jaen, Pedro
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Hosp Ramon & Cajal, Unidad Dermatol, E-28034 Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Jaen, Pedro
Batlle, Alcira
论文数: 0引用数: 0
h-index: 0
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Univ Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Univ Buenos Aires, Hosp Clin Jose San Martin, Buenos Aires, ArgentinaUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Batlle, Alcira
Stockert, Juan C.
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Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, Spain
CSIC, CIB, Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Stockert, Juan C.
Juarranz, Angeles
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Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
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Hong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R China
Chu, Ellie Shihng Meir
Wong, Thomas Kwok Shing
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Hong Kong Polytech Univ, Fac Hlth & Social Sci, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R China
Wong, Thomas Kwok Shing
Yow, Christine Miu Ngan
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Hong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R China
机构:
Acad Nacl Med Buenos Aires, Inst Estudios Oncol, RA-3092 Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Vanzulli, Silvia
Perotti, Christian
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h-index: 0
机构:
Univ Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Univ Buenos Aires, Hosp Clin Jose de San Martin, Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Perotti, Christian
Mamome, Leandro
论文数: 0引用数: 0
h-index: 0
机构:
Univ Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Univ Buenos Aires, Hosp Clin Jose de San Martin, Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Mamome, Leandro
Rodriguez, Lorena
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h-index: 0
机构:
Univ Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Univ Buenos Aires, Hosp Clin Jose de San Martin, Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Rodriguez, Lorena
Simian, Marina
论文数: 0引用数: 0
h-index: 0
机构:
Inst Angel H Roffo, RA-5481 Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Simian, Marina
Juarranz, Angeles
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h-index: 0
机构:
Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, SpainUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Juarranz, Angeles
Pontiggia, Osvaldo
论文数: 0引用数: 0
h-index: 0
机构:
Inst Angel H Roffo, RA-5481 Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Pontiggia, Osvaldo
Hasan, Tayyaba
论文数: 0引用数: 0
h-index: 0
机构:
Harvard Univ, Massachusetts Gen Hosp, Sch Med, Dept Dermatol,Wellman Labs Photomed WEL 224, Boston, MA 02114 USAUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Hasan, Tayyaba
Batlle, Alcira
论文数: 0引用数: 0
h-index: 0
机构:
Univ Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
Univ Buenos Aires, Hosp Clin Jose de San Martin, Buenos Aires, DF, ArgentinaUniv Buenos Aires, CONICET, CIPYP, Buenos Aires, DF, Argentina
机构:
Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Sanz-Rodriguez, Francisco
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机构:
Di Venosa, Gabriela
Rodriguez, Lorena
论文数: 0引用数: 0
h-index: 0
机构:
Univ Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Univ Buenos Aires, Hosp Clin Jose San Martin, Buenos Aires, ArgentinaUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Rodriguez, Lorena
Mamone, Leandro
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h-index: 0
机构:
Univ Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Univ Buenos Aires, Hosp Clin Jose San Martin, Buenos Aires, ArgentinaUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Mamone, Leandro
Blazquez, Alfonso
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h-index: 0
机构:
Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Blazquez, Alfonso
Jaen, Pedro
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Hosp Ramon & Cajal, Unidad Dermatol, E-28034 Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Jaen, Pedro
Batlle, Alcira
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h-index: 0
机构:
Univ Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Univ Buenos Aires, Hosp Clin Jose San Martin, Buenos Aires, ArgentinaUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Batlle, Alcira
Stockert, Juan C.
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h-index: 0
机构:
Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, Spain
CSIC, CIB, Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
Stockert, Juan C.
Juarranz, Angeles
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h-index: 0
机构:
Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, SpainUniv Buenos Aires, CIPYP, CONICET, Buenos Aires, Argentina
机构:
Hong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R China
Chu, Ellie Shihng Meir
Wong, Thomas Kwok Shing
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h-index: 0
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Hong Kong Polytech Univ, Fac Hlth & Social Sci, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R China
Wong, Thomas Kwok Shing
Yow, Christine Miu Ngan
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Hong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R ChinaHong Kong Polytech Univ, Dept Hlth Technol & Informat, Med Biol Sci, Hong Kong, Hong Kong, Peoples R China