Modulation of migratory activity and invasiveness of human glioma spheroids following 5-aminolevulinic acid based photodynamic treatment Laboratory investigation

被引:41
作者
Etminan, Nima [1 ]
Peters, Corinna [1 ,2 ]
Ficnar, Julian [1 ]
Anlasik, Suzan [1 ]
Buenemann, Erich [3 ]
Slotty, Philipp J. [1 ]
Haenggi, Daniel [1 ]
Steiger, Hans-Jakob [1 ]
Sorg, Rudiger V. [2 ]
Stummer, Walter [1 ,4 ]
机构
[1] Univ Dusseldorf, Dept Neurosurg, D-40225 Dusseldorf, Germany
[2] Univ Dusseldorf, Inst Transplantat Diagnost & Cell Therapeut, D-40225 Dusseldorf, Germany
[3] Univ Dusseldorf, Dept Dermatol, D-40225 Dusseldorf, Germany
[4] Univ Munster, Dept Neurosurg, D-4400 Munster, Germany
关键词
aminolevulinic acid; glioma; photodynamic therapy; invasion; migration; oncology; PHASE-III; MALIGNANT GLIOMA; GLIOBLASTOMA-MULTIFORME; ADJUVANT TEMOZOLOMIDE; CELLS RESISTANT; GENE-THERAPY; STEM-CELLS; ALA-PDT; CYTOSKELETON; RESECTION;
D O I
10.3171/2011.3.JNS10434
中图分类号
R74 [神经病学与精神病学];
学科分类号
摘要
Object. Five-aminolevulinic acid mediated photodynamic therapy (ALA/PDT) can improve the clinical outcome in patients suffering from glioblastoma. Besides direct phototoxicity, additional mechanisms may contribute. Therefore, the authors studied the influence of ALA/PDT on glioblastoma's migratory and invasive behavior in a human glioma cell spheroid model. Methods. Glioma spheroids were grown from human U373 and A172 cell lines. After ALA/PDT of spheroids, the authors assessed the migration of tumor cells and their capacity to invade a collagen matrix, as well as changes in their viability, morphology, and expression of matrix metalloproteinases (MMPs). Results. The authors found that ALA/PDT caused long-lasting, nearly complete suppression of glioma cell migration and matrix invasion compared with nontherapeutic controls, including either irradiation or incubation with ALA only. Although ALA/PDT induced tumor cell apoptosis, suppression of migration/invasion was not simply due to phototoxicity because 50% of tumor cells remained vital throughout the observation period. Moreover, the morphology of ALA/PDT-treated cells changed significantly toward a polygonal, epithelial-like appearance, which was associated with alterations in the actin cytoskeleton. Furthermore, downregulation of MMP-7 and -8 was observed after treatment whereas other MMPs remained unchanged. Conclusions. In addition to directly eliminating glioma cells through apoptosis. ALA/PDT alters their invasiveness, possibly due to the effects on the cytoskeletal organization and MMP expression. (DOI: 10.3171/2011.3.JNS10434)
引用
收藏
页码:281 / 288
页数:8
相关论文
共 31 条
  • [1] Differential effects of photofrin, 5-aminolevulinic acid and calphostin C on glioma cells
    Au, Cheuk Man
    Luk, Sze Ki
    Jackson, Christopher John
    Ng, Ho Keung
    Yow, Christine Miu Ngan
    To, Shing Shun Tony
    [J]. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, 2006, 85 (02) : 92 - 101
  • [2] Interstitial photodynamic therapy of nonresectable malignant glioma recurrences using 5-aminolevulinic acid induced protoporphyrin IX
    Beck, Tobias J.
    Kreth, Friedrich W.
    Beyer, Wolfgang
    Mehrkens, Jan H.
    Obermeier, Andreas
    Stepp, Herbert
    Stummer, Walter
    Baumgartner, Reinhold
    [J]. LASERS IN SURGERY AND MEDICINE, 2007, 39 (05) : 386 - 393
  • [3] Proteases and the biology of glioma invasion
    Binder, DK
    Berger, MS
    [J]. JOURNAL OF NEURO-ONCOLOGY, 2002, 56 (02) : 149 - 158
  • [4] Decreased metastatic phenotype in cells resistant to aminolevulinic acid-photodynamic therapy
    Casas, Adriana
    Di Venosa, Gabriela
    Vanzulli, Silvia
    Perotti, Christian
    Mamome, Leandro
    Rodriguez, Lorena
    Simian, Marina
    Juarranz, Angeles
    Pontiggia, Osvaldo
    Hasan, Tayyaba
    Batlle, Alcira
    [J]. CANCER LETTERS, 2008, 271 (02) : 342 - 351
  • [5] Disorganisation of cytoskeleton in cells resistant to photodynamic treatment with decreased metastatic phenotype
    Casas, Adriana
    Sanz-Rodriguez, Francisco
    Di Venosa, Gabriela
    Rodriguez, Lorena
    Mamone, Leandro
    Blazquez, Alfonso
    Jaen, Pedro
    Batlle, Alcira
    Stockert, Juan C.
    Juarranz, Angeles
    [J]. CANCER LETTERS, 2008, 270 (01) : 56 - 65
  • [6] Photodynamic therapy and anti-tumour immunity
    Castano, Ana P.
    Mroz, Pawel
    Hamblin, Michael R.
    [J]. NATURE REVIEWS CANCER, 2006, 6 (07) : 535 - 545
  • [7] Photodynamic effect in medulloblastoma: downregulation of matrix metalloproteinases and human telomerase reverse transcriptase expressions
    Chu, Ellie Shihng Meir
    Wong, Thomas Kwok Shing
    Yow, Christine Miu Ngan
    [J]. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2008, 7 (01) : 76 - 83
  • [8] Photodynamic therapy for cancer
    Dolmans, DEJGJ
    Fukumura, D
    Jain, RK
    [J]. NATURE REVIEWS CANCER, 2003, 3 (05) : 380 - 387
  • [9] ALA and Photofrin® fluorescence-guided resection and repetitive PDT in glioblastoma multiforme:: a single centre Phase III randomised controlled trial
    Eljamel, M. Sam
    Goodman, Carol
    Moseley, Harry
    [J]. LASERS IN MEDICAL SCIENCE, 2008, 23 (04) : 361 - 367
  • [10] Epidemiology of brain tumors
    Fisher, James L.
    Schwartzbaum, Judith A.
    Wrensch, Margaret
    Wiemels, Joseph L.
    [J]. NEUROLOGIC CLINICS, 2007, 25 (04) : 867 - +