Catalytic synthesis and antitumor activities of sulfated polysaccharide from Gynostemma pentaphyllum Makino

被引：54

作者：

Chen, Tong ^{[1
]}

Li, Bo ^{[2
]}

Li, Yuanyuan ^{[1
]}

Zhao, Chuande ^{[1
]}

Shen, Jianmin ^{[2
]}

Zhang, Haixia ^{[1
]}

机构：

[1] Lanzhou Univ, State Key Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

[2] Lanzhou Univ, Coll Life Sci, Inst Canc Biol & Drug Screening, Lanzhou 730000, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2011年 / 83卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Gynostemma pentaphyllum Makino; Sulfated derivatives; Ionic liquid; Catalyst; Antitumor activity; IN-VITRO; PLEUROTUS-OSTREATUS; BETA-GLUCAN; DERIVATIVES; ELUCIDATION; LIQUID;

D O I：

10.1016/j.carbpol.2010.08.024

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A native polysaccharides (GPP2) was isolated from Gynostemma pentaphyllum Makino Four sulfated derivatives of GPP2 (GPP2-s1 to s4) were prepared by chlorosulfonic acid method with ionic liquids (ILs) as solvent and 4-dimethylaminopyridine (DMAP) as catalyst The structures of the sulfated derivatives were analyzed by FT-IR spectra and C-13 NMR spectra which indicated that the sulfated groups were introduced mainly at the C-6 position as well as at the C-2 position The products showed different degree of substitution (DS) ranging from 0 79 to 1 34 and different weight-average molecular mass (Mw) ranging from 8 64 to 11 2 Da The sulfated derivatives inhibited the growth of HepG2 cells and Hela cells in vitro significantly Furthermore they had no obvious influence on 293 cells which indicated that they had low toxicity Flow cytometric studies revealed that treatment of the sulfated derivatives with Hela cells could mediate the cell-cycle arrest in the S phase (C) 2010 Elsevier Ltd All rights reserved

引用

页码：554 / 560

页数：7

共 30 条

[1] GAS-LIQUID-CHROMATOGRAPHY MASS-SPECTROMETRY ANALYSIS OF ANTICOAGULANT ACTIVE CURDLAN SULFATES [J].

ALBAN, S ;

FRANZ, G .

SEMINARS IN THROMBOSIS AND HEMOSTASIS, 1994, 20 (02) :152-158

[2]

BIRGITTE LC, 1995, J ETHNOPHARMACOL, V46, P125

[3]

Cai T. S., 2005, J CLIN REHABILITATIO, V9, P106

[4] Effect of (1→3)- and (1→4)-linkages of fully sulfated polysaccharides on their anticoagulant activity [J].

Chaidedgumjorn, A ;

Toyoda, H ;

Woo, ER ;

Lee, KB ;

Kim, YS ;

Toida, T ;

Imanari, T .

CARBOHYDRATE RESEARCH, 2002, 337 (10) :925-933

[5]

GAMAZADE A, 1997, CARBOHYD POLYM, V34, P113

[6] Alternative medium for production of Pleurotus ostreatus biomass and potential antitumor polysaccharides [J].

Gern, Regina Maria Miranda ;

Wisbeck, Elisabeth ;

Rosa, Jamile Rampinelli ;

Ninow, Jorge Luiz ;

Furlan, Sandra Aparecida .

BIORESOURCE TECHNOLOGY, 2008, 99 (01) :76-82

[7] Structural characteristics and antioxidant activities of the extracellular polysaccharides produced by marine bacterium Edwardsiella tarda [J].

Guo, Shoudong ;

Mao, Wenjun ;

Han, Yin ;

Zhang, Xiaohua ;

Yang, Chunli ;

Chen, Yin ;

Chen, Yanli ;

Xu, Jian ;

Li, Hongyan ;

Qi, Xiaohui ;

Xu, Jiachao .

BIORESOURCE TECHNOLOGY, 2010, 101 (12) :4729-4732

[8] New triterpenoid saponins from Gynostemma pentaphyllum [J].

Hu, LH ;

Chen, ZL ;

Xie, YY .

JOURNAL OF NATURAL PRODUCTS, 1996, 59 (12) :1143-1145

[9] Molecular mass and antitumor activities of sulfated derivatives of α-glucan from Poria cocos mycelia [J].

Lin, YL ;

Zhang, LN ;

Chen, L ;

Jin, Y ;

Zeng, FB ;

Jin, J ;

Wan, B ;

Cheung, PCK .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2004, 34 (05) :289-294

[10] Sulfation of a polysaccharide obtained from Phellinus ribis and potential biological activities of the sulfated derivatives [J].

Liu, Yuhong ;

Liu, Chunhui ;

Tan, Haining ;

Zhao, Ting ;

Cao, Jichao ;

Wang, Fengshan .

CARBOHYDRATE POLYMERS, 2009, 77 (02) :370-375

← 1 2 3 →