Inhibition of α, βI, δ, η and ζ protein kinase C isoforms by xanthonolignoids

被引：23

作者：

Saraiva, L

Fresco, P

Pinto, E

Sousa, E

Pinto, M

Gonçalves, J

机构：

[1] Univ Porto, Fac Farm, CEQOFFUP, Serv Farmacol, P-4050047 Oporto, Portugal

[2] Univ Porto, Fac Farm, CEQOFFUP, Microbiol Serv, P-4050047 Oporto, Portugal

[3] Univ Porto, Fac Farm, CEQOFFUP, Serv Quim Organ, P-4050047 Oporto, Portugal

来源：

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY | 2003年 / 18卷 / 04期

关键词：

protein kinase C; PKC isoforms; yeast phenotypic assay; PKC inhibitors; xanthonolignoids; kielcorins;

D O I：

10.1080/147563601000118400

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of the xanthonolignoids trans -(+/-)-kielcorin C, cis -(+/-)-kielcorin C, trans -(+/-)-kielcorin D, trans -(+/-)-isokielcorin D and trans -(+/-)-kielcorin E on isoforms alpha, betaI, delta, eta and zeta of protein kinase C (PKC) was studied using the yeast phenotypic assay. All the compounds tested revealed an effect compatible with PKC inhibition, similar to that exhibited by the well established PKC inhibitor chelerythrine, and with differences in their potency towards the distinct isoforms tested, being, in general, potent inhibitors of the atypical PKC isoform (PKC-zeta). PKC inhibition caused by these kielcorins was confirmed using an in vitro kinase assay. The present study constitutes the first attempt to unravel the molecular mechanism of kielcorins activity, and shows that xanthonolignoids are a promising group of compounds to investigate for isoform selective PKC inhibitors.

引用

页码：357 / 370

页数：14

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[11] The role of protein kinase C isoforms in insulin action [J].

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