Optimization of two-dimensional gel electrophoresis protocols for Boesenbergia rotunda in vitro suspension culture

被引：0

作者：

Chong, Tan Eng ^{[1
,4
]}

Teck, Foo Gen ^{[3
,5
]}

Ming, Wong Sher ^{[3
,5
]}

Abd Rahman, Noorsaadah ^{[2
,4
]}

Khalid, Norzulaani ^{[3
,5
]}

Karsani, Saiful Anuar ^{[3
,4
]}

Othman, Shatrah ^{[1
,4
]}

Yusof, Rohana ^{[1
,4
]}

机构：

[1] Univ Malaya, Fac Med, Dept Mol Med, Kuala Lumpur 50603, Malaysia

[2] Univ Malaya, Fac Sci, Dept Chem, Kuala Lumpur 50603, Malaysia

[3] Univ Malaya, Fac Sci, Inst Biol Sci, Kuala Lumpur 50603, Malaysia

[4] Univ Malaya, Drug Design & Dev Res Grp, Kuala Lumpur 50603, Malaysia

[5] Univ Malaya, Biotechnol & Bioprod Res Cluster UMBIO, Kuala Lumpur 50603, Malaysia

来源：

JOURNAL OF MEDICINAL PLANTS RESEARCH | 2011年 / 5卷 / 16期

关键词：

Two dimensional electrophoresis; plant proteomics; Boesenbergia rotunda; Temu kunci; callus; PROTEOMICS; FINGERROOT; PROTEINS; L;

D O I：

暂无

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Boesenbergia rotunda belongs to the Zingiberaceae family and is abundant in the Southeast Asia. It is widely used as food ingredient and traditional medicine. Biologically, the plant extract contains pharmaceutically important bioactive compounds that exhibit anti-HIV protease, antibacterial, antifungal, anti-inflammatory, anti-tumor and antioxidant activities. Proteomics approaches to study the proteins and/or enzymes involved in the biosynthesis of these compounds are challenging due to the complexity of plant samples and the presence of interfering substances. Here, we describe the development a highly robust and reproducible two-dimensional gel electrophoresis (2DE) protocols for resolving the proteome of B. rotunda suspension cultures.

引用

页码：3777 / 3780

页数：4

共 17 条

[1]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[2] Focus on plant proteomics [J].