Topological Relationships Cytoskeleton-Membrane Nanosurface-Morphology as a Basic Mechanism of Total Disorders of RBC Structures

被引：5

作者：

Kozlova, Elena ^{[1
,2
,3
]}

Sergunova, Viktoria ^{[1
]}

Sherstyukova, Ekaterina ^{[1
,2
]}

Gudkova, Olga ^{[1
]}

Kozlov, Aleksandr ^{[2
]}

Inozemtsev, Vladimir ^{[1
]}

Lyapunova, Snezhanna ^{[1
]}

Chernysh, Aleksandr ^{[1
]}

机构：

[1] VA Negovsky Res Inst Gen Reanimatol, Fed Res & Clin Ctr Intens Care Med & Rehabilitol, Lab Biophys Cell Membranes Crit State, Moscow 107031, Russia

[2] Sechenov First Moscow State Med Univ, Dept Med & Biol Phys, Sechenov Univ, Moscow 119991, Russia

[3] MV Lomonosov Moscow State Univ Lomonosov MSU, Fac Phys, Fed State Budget Educ Inst Higher Educ, Moscow 119234, Russia

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2022年 / 23卷 / 04期

关键词：

red blood cells; cytoskeleton; membrane nanosurface; structure disorder; atomic force microscopy; hemin; ultraviolet radiation; temperature changes; pH; oxidative processes; RED-BLOOD-CELLS; OXIDATIVE STRESS; DAMAGE; SPECTRIN; STORAGE; NANOSTRUCTURE; RADIATION; BAND-3; HEMIN;

D O I：

10.3390/ijms23042045

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The state of red blood cells (RBCs) and their functional possibilities depend on the structural organization of the membranes. Cell morphology and membrane nanostructure are compositionally and functionally related to the cytoskeleton network. In this work, the influence of agents (hemin, endogenous oxidation during storage of packed RBCs, ultraviolet (UV) radiation, temperature, and potential of hydrogen (pH) changes) on the relationships between cytoskeleton destruction, membrane nanostructure, and RBC morphology was observed by atomic force microscope. It was shown that the influence of factors of a physical and biochemical nature causes structural rearrangements in RBCs at all levels of organization, forming a unified mechanism of disturbances in relationships "cytoskeleton-membrane nanosurface-cell morphology". Filament ruptures and, consequently, large cytoskeleton pores appeared. The pores caused membrane topological defects in the form of separate grain domains. Increasing loading doses led to an increase in the number of large cytoskeleton pores and defects and their fusion at the membrane nanosurfaces. This caused the changes in RBC morphology. Our results can be used in molecular cell biology, membrane biophysics, and in fundamental and practical medicine.

引用

页数：16