An Advance in Proline Ligation

被引：93

作者：

Shang, Shiying ^{[1
]}

Tan, Zhongping ^{[1
]}

Dong, Suwei ^{[1
]}

Danishefsky, Samuel J. ^{[1
,2
]}

机构：

[1] Sloan Kettering Inst Canc Res, Bioorgan Chem Lab, New York, NY 10065 USA

[2] Columbia Univ, Dept Chem, New York, NY 10027 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2011年 / 133卷 / 28期

关键词：

NATIVE CHEMICAL LIGATION; PROTEIN-SYNTHESIS; GLYCOPEPTIDE SYNTHESIS; THIYL RADICALS; PEPTIDE; CYSTEINE; DESULFURIZATION; RECOGNITION; EFFICIENT; INSIGHTS;

D O I：

10.1021/ja204277b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Native chemical ligation (NCL) is widely applicable for building proteins in the laboratory. Since the discovery of this method, many strategies have been developed to enhance its capability and efficiency. Because of the poor reactivity of proline thioesters, ligation at a C-terminal proline site is not readily accomplished. Here, we demonstrate that ligation at an N-terminal protein is feasible using the combined logic of NCL and metal-free dethiylation (MFD).

引用

页码：10784 / 10786

页数：3

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