Evolution of genome size across some cultivated Allium species

被引:75
作者
Ricroch, A
Yockteng, R
Brown, SC
Nadot, S
机构
[1] Univ Paris 11, Lab Ecol Syst & Evolut, CNRS, UMR 8079, F-91405 Orsay, France
[2] CNRS, Inst Sci Vegetal, Lab Cytometrie, UPR 2355, F-91198 Gif Sur Yvette, France
关键词
Allium; nuclear DNA amount; GC content; flow cytometry; internal transcribed spacer (ITS); phylogeny; life form;
D O I
10.1139/G05-017
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Allium L. (Alliaceae), a genus of major economic importance, exhibits a great diversity in various morphological characters and particularly in life form, with bulbs and rhizomes. Allium species show variation in several cytogenetic characters such as basic chromosome number, ploidy level, and genome size. The purpose of the present investigation was to study the evolution of nuclear DNA amount, GC content, and life form. A phylogenetic approach was used on a sample of 30 Allium species, including major vegetable crops and their wild allies, belonging to the 3 major subgenera Allium, Amerallium, and Rhizirideum and 14 sections. A phylogeny was constructed using internal transcribed spacer (ITS) sequences of 43 accessions representing 30 species, and the nuclear DNA amount and the GC content of 24 Allium species were investigated by flow cytometry. For the first time, the nuclear DNA content of Allium cyaneum and Allium vavilovii was measured, and the GC content of 16 species was measured. We addressed the following questions: (i) Is the variation in nuclear DNA amount and GC content linked to the evolutionary history of these edible Allium species and their wild relatives? (ii) How did life form (rhizome or bulb) evolve in edible Allium? Our results revealed significant interspecific variation in the nuclear DNA amount as well as in the GC content. No correlation was found between the GC content and the nuclear DNA amount. The reconstruction of nuclear DNA amount on the phylogeny showed a tendency towards a decrease in genome size within the genus. The reconstruction of life form history showed that rhizomes evolved in the subgenus Rhizirideum from an ancestral bulbous life form and were subsequently lost at least twice independently in this subgenus.
引用
收藏
页码:511 / 520
页数:10
相关论文
共 46 条
[1]   Genome size in Allium:: In quest of reproducible data [J].
Baranyi, M ;
Greilhuber, J .
ANNALS OF BOTANY, 1999, 83 (06) :687-695
[2]   Lack of correlation between AT frequency and genome size in higher plants and the effect of nonrandomness of base sequences on dye binding [J].
Barow, M ;
Meister, A .
CYTOMETRY, 2002, 47 (01) :1-7
[3]   Interspecific chromosomal rearrangements in monosomic addition lines of Allium [J].
Barthes, L ;
Ricroch, A .
GENOME, 2001, 44 (05) :929-935
[4]   Nuclear DNA amounts in angiosperms and their modern uses - 807 new estimates [J].
Bennett, MD ;
Bhandol, P ;
Leitch, IJ .
ANNALS OF BOTANY, 2000, 86 (04) :859-909
[5]   NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].
BENNETT, MD ;
SMITH, JB .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274
[6]  
CHEREMUSHKINA VA, 1992, EVOLUTION LIFE FORMS, P27
[7]   Cytology of the highly polyploid disjunct species, Allium dregeanum (Alliaceae), and of some Eurasian relatives [J].
DeSarker, D ;
Johnson, MAT ;
Reynolds, A ;
Brandham, PE .
BOTANICAL JOURNAL OF THE LINNEAN SOCIETY, 1997, 124 (04) :361-373
[8]   Genome size reduction through illegitimate recombination counteracts genome expansion in Arabidopsis [J].
Devos, KM ;
Brown, JKM ;
Bennetzen, JL .
GENOME RESEARCH, 2002, 12 (07) :1075-1079
[9]   Molecular and morphological evidence for an origin of the aberrant genus Milula within Himalayan species of Allium (Alliacae) [J].
Friesen, N ;
Fritsch, RM ;
Pollner, S ;
Blattner, FR .
MOLECULAR PHYLOGENETICS AND EVOLUTION, 2000, 17 (02) :209-218
[10]   Allotetraploid origin of Allium altyncolicum (Alliaceae, Allium sect Schoenoprasum) as investigated by karyological and molecular markers [J].
Friesen, N ;
Borisjuk, N ;
Mes, THM ;
Klaas, M ;
Hanelt, P .
PLANT SYSTEMATICS AND EVOLUTION, 1997, 206 (1-4) :317-335