A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells

被引：44
作者：
Borras-Linares, Isabel [1 ,3 ]
Perez-Sanchez, Almudena [2 ]
Lozano-Sanchez, Jesus [1 ,3 ]
Barrajon-Catalan, Enrique [2 ]
Arraez-Roman, David [1 ,3 ]
Cifuentes, Alejandro [4 ]
Micol, Vicente [2 ,5 ]
Segura Carretero, Antonio [1 ,3 ]
机构：
[1] Univ Granada, Fac Sci, Dept Analyt Chem, E-18071 Granada, Spain
[2] Miguel Hernandez Univ, Inst Mol & Cellular Biol, Elche 03202, Spain
[3] Hlth Sci Technol Pk, Res & Dev Funct Food Ctr CIDAF, Granada 18100, Spain
[4] CSIC, CIAL, Lab Food, E-28049 Madrid, Spain
[5] Inst Salud Carlos III, CIBER, CIBERobn, Fisiopatol Obesidad & Nutr,CB12 03 30038, Madrid, Spain
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2015年 / 80卷
关键词：
Antiproliferative activity; Cytotoxicity; Colon cancer; HPLC-ESI-QTOF-MS; Rosemary; Terpenoids; ROSMARINUS-OFFICINALIS L; SUPERCRITICAL-FLUID EXTRACTS; MASS SPECTROMETRIC ANALYSIS; CARNOSIC ACID; SALVIA-OFFICINALIS; URSOLIC ACID; ANTIOXIDANT ACTIVITIES; SIGNALING PATHWAY; QUERCETIN; POLYPHENOLS;
D O I：
10.1016/j.fct.2015.03.013
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Rosemary extracts have exhibited potential cytostatic or cytotoxic effects in several cancer cell models but their bioactive compounds are yet to be discovered. In this work, the anticancer activity of a rosemary-leaf extract and its fractions were assayed to identify the phenolic compounds responsible for their antiproliferative/cytotoxic effects on a panel of human colon cancer cell lines. Bioguided fractionation of the rosemary-leaf extract was achieved by semi-preparative chromatography. The rosemary extract and the compounds in the fractions were characterized and quantified by HPLC-ESI-QTOF-MS. Cellular viability in the presence of these fractions and the whole extract was determined after 24 or 48 h incubations by using an MU assay. Fractions containing diterpenes or triterpenes were the most active but not as much as the whole extract. In conclusion, camosic acid, carnosol, 12-methoxycamosic acid, taxodione, hinokione and betulinic acid were the putative candidates that contributed to the observed antiproliferative activity of rosemary in human colon cancer cells. Whether the effects of the extract and fractions are only cytostatic or cytotoxic needs to be elucidated. Nevertheless, the comparative antiproliferative study on the fractions and whole extract revealed potential synergistic effects between several components in the extract that may deserve further attention. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：215 / 222
页数：8
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