A bioguided identification of the active compounds that contribute to the antiproliferative/cytotoxic effects of rosemary extract on colon cancer cells

被引：44
作者：
Borras-Linares, Isabel [1 ,3 ]
Perez-Sanchez, Almudena [2 ]
Lozano-Sanchez, Jesus [1 ,3 ]
Barrajon-Catalan, Enrique [2 ]
Arraez-Roman, David [1 ,3 ]
Cifuentes, Alejandro [4 ]
Micol, Vicente [2 ,5 ]
Segura Carretero, Antonio [1 ,3 ]
机构：
[1] Univ Granada, Fac Sci, Dept Analyt Chem, E-18071 Granada, Spain
[2] Miguel Hernandez Univ, Inst Mol & Cellular Biol, Elche 03202, Spain
[3] Hlth Sci Technol Pk, Res & Dev Funct Food Ctr CIDAF, Granada 18100, Spain
[4] CSIC, CIAL, Lab Food, E-28049 Madrid, Spain
[5] Inst Salud Carlos III, CIBER, CIBERobn, Fisiopatol Obesidad & Nutr,CB12 03 30038, Madrid, Spain
来源：
FOOD AND CHEMICAL TOXICOLOGY | 2015年 / 80卷
关键词：
Antiproliferative activity; Cytotoxicity; Colon cancer; HPLC-ESI-QTOF-MS; Rosemary; Terpenoids; ROSMARINUS-OFFICINALIS L; SUPERCRITICAL-FLUID EXTRACTS; MASS SPECTROMETRIC ANALYSIS; CARNOSIC ACID; SALVIA-OFFICINALIS; URSOLIC ACID; ANTIOXIDANT ACTIVITIES; SIGNALING PATHWAY; QUERCETIN; POLYPHENOLS;
D O I：
10.1016/j.fct.2015.03.013
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Rosemary extracts have exhibited potential cytostatic or cytotoxic effects in several cancer cell models but their bioactive compounds are yet to be discovered. In this work, the anticancer activity of a rosemary-leaf extract and its fractions were assayed to identify the phenolic compounds responsible for their antiproliferative/cytotoxic effects on a panel of human colon cancer cell lines. Bioguided fractionation of the rosemary-leaf extract was achieved by semi-preparative chromatography. The rosemary extract and the compounds in the fractions were characterized and quantified by HPLC-ESI-QTOF-MS. Cellular viability in the presence of these fractions and the whole extract was determined after 24 or 48 h incubations by using an MU assay. Fractions containing diterpenes or triterpenes were the most active but not as much as the whole extract. In conclusion, camosic acid, carnosol, 12-methoxycamosic acid, taxodione, hinokione and betulinic acid were the putative candidates that contributed to the observed antiproliferative activity of rosemary in human colon cancer cells. Whether the effects of the extract and fractions are only cytostatic or cytotoxic needs to be elucidated. Nevertheless, the comparative antiproliferative study on the fractions and whole extract revealed potential synergistic effects between several components in the extract that may deserve further attention. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：215 / 222
页数：8
相关论文
共 54 条
  • [1] Liquid chromatograpic-mass spectrometric analysis of phenolics and free radical scavenging activity of rosemary extract from different raw material
    Almela, Luis
    Sanchez-Munoz, Blas
    Fernandez-Lopez, Jose A.
    Roca, Maria J.
    Rabe, Virginia
    [J]. JOURNAL OF CHROMATOGRAPHY A, 2006, 1120 (1-2) : 221 - 229
  • [2] Characterization of topical antiinflammatory compounds in Rosmarinus officinalis L.
    Altinier, Gianmario
    Sosa, Silvio
    Aquino, Rita P.
    Mencherini, Teresa
    Della Loggia, Roberto
    Tubaro, Aurelia
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (05) : 1718 - 1723
  • [3] Flavonoids and Phenolic Compounds from Rosmarinus officinalis
    Bai, Naisheng
    He, Kan
    Roller, Marc
    Lai, Ching-Shu
    Shao, Xi
    Pan, Min-Hsiung
    Ho, Chi-Tang
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2010, 58 (09) : 5363 - 5367
  • [4] In vivo assessment of antidiabetic and antioxidant activities of rosemary (Rosmarinus officinalis) in alloxan-diabetic rabbits
    Bakirel, Tuelay
    Bakirel, Utku
    Keles, Oya Uestuener
    Ulgen, Sinem Gunes
    Yardibi, Hasret
    [J]. JOURNAL OF ETHNOPHARMACOLOGY, 2008, 116 (01) : 64 - 73
  • [5] Carnosic acid inhibits the proliferation and migration capacity of human colorectal cancer cells
    Barni, M. V.
    Carlini, M. J.
    Cafferata, E. G.
    Puricelli, L.
    Moreno, S.
    [J]. ONCOLOGY REPORTS, 2012, 27 (04) : 1041 - 1048
  • [6] Molecular Promiscuity of Plant Polyphenols in the Management of Age-Related Diseases: Far Beyond Their Antioxidant Properties
    Barrajon-Catalan, Enrique
    Herranz-Lopez, Maria
    Joven, Jorge
    Segura-Carretero, Antonio
    Alonso-Villaverde, Carlos
    Menendez, Javier A.
    Micol, Vicente
    [J]. OXIDATIVE STRESS AND INFLAMMATION IN NON-COMMUNICABLE DISEASES - MOLECULAR MECHANISMS AND PERSPECTIVES IN THERAPEUTICS, 2014, 824 : 141 - 159
  • [7] Borras LI, 2011, J CHROMATOGR A, V1218, P7682, DOI DOI 10.1016/J.CHR0MA.2011.07.021
  • [8] Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., lamiaceae) essential oils
    Bozin, Biljana
    Mlmica-Dukic, Neda
    Samojlik, Isidora
    Jovin, Emilija
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2007, 55 (19) : 7879 - 7885
  • [9] Qualitative and quantitative analysis of active flavonoids in Flos Lonicerae by capillary zone electrophoresis coupled with solid-phase extraction
    Chen, J
    Li, SL
    Li, P
    Song, Y
    Chai, XY
    Ma, DY
    [J]. JOURNAL OF SEPARATION SCIENCE, 2005, 28 (04) : 365 - 372
  • [10] Chen XG, 2006, J PHARMACEUT BIOMED, V40, P559, DOI 10.1016/j.jpba.2005.07.043
123456