Comparison of DNA pyrosequencing with alternative methods for identification of mycobacteria

被引：18

作者：

Heller, Loree C. ^{[1
]}

Jones, Michael ^{[2
]}

Widen, Ray H. ^{[1
]}

机构：

[1] Tampa Gen Hosp, Dept Pathol, Tampa, FL 33601 USA

[2] Sacred Heart Hosp, Microbiol Lab, Pensacola, FL 32504 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2008年 / 46卷 / 06期

关键词：

D O I：

10.1128/JCM.02001-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Identification of mycobacterial clinical isolates by pyrosequencing within the hypervariable A region of the 16S rRNA gene was compared to other identification methods. For > 90% of isolates, these identifications correlated to the level of complex or species. For identification of many mycobacteria, pyrosequencing offers an inexpensive alternative to traditional sequencing.

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页码：2092 / 2094

页数：3

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