Optimal time point for the transplantation of neural stem cells induced to differentiate with retinoic acid

被引：0
|
作者：
Wang, Shuxin [1 ]
Pan, Dengji [1 ]
Liu, Na [1 ]
Liu, Yongming [1 ]
Chen, Juan [1 ]
Ni, Houjie [1 ]
Tang, Zhouping [1 ]
机构：
[1] Huazhong Univ Sci & Technol, Dept Neurol, Tongji Hosp, Tongji Med Coll, Wuhan 430030, Hubei Province, Peoples R China
来源：
NEURAL REGENERATION RESEARCH | 2011年 / 6卷 / 16期
基金：
中国国家自然科学基金;
关键词：
neural stem cells; retinoic acid; artificial cerebrospinal fluid; induced differentiation; cell transplantation; optimal time point; neural regeneration; CENTRAL-NERVOUS-SYSTEM; PROMOTES FUNCTIONAL RECOVERY; ADULT-RAT BRAIN; PROGENITOR CELLS; PRECURSOR CELLS; NEURONAL DIFFERENTIATION; INTRACEREBRAL HEMORRHAGE; STROKE MODEL; CNS; NEUROGENESIS;
D O I：
10.3969/j.issn.1673-5374.2011.16.008
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Previous studies have demonstrated that differentiated neural stem cells (NSCs) are more suitable for transplantation than non-differentiated NSCs. In this study, NSCs were expanded in vitro for two passages, induced with retinoic acid to differentiate, and harvested between 1-6 days later. They were subsequently cultured in artificial cerebrospinal fluid for an additional 3 days, during which their growth and morphology was monitored. NSCs induced for 4 days exhibited a peak rate of cells differentiating into neurons and robust growth. Our results indicate that the optimal time point for transplanting NSCs is following a 4-day period of induced differentiation.
引用
收藏
页码：1243 / 1247
页数：5
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