Stable binding of DNA to zwitterionic lipid bilayers in aqueous solutions

被引:30
|
作者
Malghani, MS [1 ]
Yang, J [1 ]
机构
[1] Univ Vermont, Dept Phys, Burlington, VT 05405 USA
来源
JOURNAL OF PHYSICAL CHEMISTRY B | 1998年 / 102卷 / 44期
关键词
D O I
10.1021/jp982413g
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We found that DNA molecules bind strongly to zwitterionic dipalmitoylphosphatidylcholine lipid bilayers in solutions, facilitating in situ imaging with an atomic force microscope. The binding strength has a lower limit of about 2 kT per helical turn with kT the thermal energy at room temperature, The height of membrane-bound DNA is the same as the diameter of DNA, indicating no compression by the tip at a probe force of about 0.1 nN.
引用
收藏
页码:8930 / 8933
页数:4
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