Effects of ultrasonic processing on caspase-3, calpain expression and myofibrillar structure of chicken during post-mortem ageing

被引：56

作者：

Chen, Lin ^{[1
]}

Feng, Xian-Chao ^{[1
]}

Zhang, Ying-yang ^{[2
]}

Liu, Xue-bo ^{[1
]}

Zhang, Wan-gang ^{[3
]}

Li, Chun-bao ^{[3
]}

Ullah, Niamat ^{[1
]}

Xu, Xing-lian ^{[3
]}

Zhou, Guang-hong ^{[3
]}

机构：

[1] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Peoples R China

[2] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

[3] Nanjing Agr Univ, Coll Food Sci & Technol, Synerget Innovat Ctr Food Safety & Nutr, Key Lab Meat Proc & Qual Control,Minist Educ, Nanjing 210095, Jiangsu, Peoples R China

来源：

FOOD CHEMISTRY | 2015年 / 177卷

关键词：

Apoptosis; Caspase-3; Calpain; Post-mortem ageing; Ultrasonic processing; HIGH-POWER ULTRASOUND; FOCUSED ULTRASOUND; SKELETAL-MUSCLE; INHIBITOR; APOPTOSIS; MEAT; CALPASTATIN; PROTEOLYSIS; PROTEINS; FOOD;

D O I：

10.1016/j.foodchem.2014.11.064

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this investigation, ultrasonic treatments at a frequency of 40 kHz and power of 1500W made caspase-3 and calpain activities significantly higher in chicken muscle after slaughter during 5 d storage (p < 0.01). Additionally Western blotting analysis of a-spectrin showed that ultrasonic treatments caused the production of alpha-spectrin degradation products of 120 and 150 kDa more dense than the control (p < 0.01). Degradation of calpastatin during chicken meat ageing was induced by ultrasound treatments (p < 0.01), which suggested the ability of caspase-3 to cleave calpastatin. SDS-PAGE showed that all treatments enhanced accumulation of the 30 kDa troponin-T degradation product (p < 0.01), and transmission electron microscopy images of myofibrils displayed that damage of ultrasound treatment for 60 min was more extensive than at 30 min. The findings proved the low-frequency and high power ultrasonic treatments improved disruption of myofibrillar structures and increased proteolysis of chicken meat faster by triggering an apoptosis cascade. (C) 2015 Published by Elsevier Ltd.

引用

页码：280 / 287

页数：8

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