Exhaled 8-isoprostane in sarcoidosis: relation to superoxide anion production by bronchoalveolar lavage cells

被引：8
作者：
Piotrowski, Wojciech J. [1 ]
Kurmanowska, Zofia [1 ]
Antczak, Adam [1 ]
Marczak, Jerzy [1 ]
Ciebiada, Maciej [1 ]
Gorski, Pawel [1 ]
机构：
[1] Med Univ Lodz, Dept Pneumol & Allergy, PL-90153 Lodz, Poland
来源：
INFLAMMATION RESEARCH | 2010年 / 59卷 / 12期
关键词：
Bronchoalveolar lavage; Exhaled breath condensate; Oxidative stress; Sarcoidosis; Superoxide anion; INTERSTITIAL LUNG-DISEASES; ALVEOLAR MACROPHAGES; BREATH CONDENSATE; OXIDATIVE STRESS; PULMONARY SARCOIDOSIS; HYDROGEN-PEROXIDE; FLUID PROTEINS; II PNEUMOCYTES; BAL FLUID; RELEASE;
D O I：
10.1007/s00011-010-0222-4
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
This study was designed to examine the mutual relationship between 8-isoprostane in exhaled breath condensate (EBC) and superoxide anion generation by bronchoalveolar lavage fluid (BALF) cells in patients with sarcoidosis. About 29 patients with sarcoidosis, 34 healthy never smokers (control group for EBC) and 15 healthy never smokers (control group for BAL) were examined. EBC was collected directly before bronchoscopy. 8-Isoprostane was measured by ELISA, and superoxide anion by colorimetry. Exhaled breath condensate 8-isoprostane is increased in sarcoidosis (median, 25-75 percentile): 2.50; 2.50-3.90 versus 6.20; 2.50-16.95 pg/ml, p a parts per thousand currency sign 0.05). Spontaneous superoxide anion release from BALF cells was significantly elevated only in patients with a high percentage of lymphocytes in BALF (6.42 +/- A 1.24 vs. 23.52 +/- A 4.30 nmol/10(6) cells, p a parts per thousand currency sign 0.01). There were no correlations between 8-isoprostane and spontaneous or stimulated superoxide anion release. We confirmed higher concentrations of EBC 8-isoprostane in sarcoidosis and higher spontaneous release of superoxide anion from BALF cells in patients with sarcoidosis. The increase of EBC 8-isoprostane is not directly related to superoxide anion released from BALF cells.
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页码：1027 / 1032
页数：6
相关论文
共 27 条
[1]  
ARCOLI JJ, 2008, AM J RESP CRIT CARE, V179, P105
[2]   Antioxidative and clinical effects of high-dose N-acetylcysteine in fibrosing alveolitis - Adjunctive therapy to maintenance immunosuppression [J].
Behr, J ;
Maier, K ;
Degenkolb, B ;
Krombach, F ;
Vogelmeier, C .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1997, 156 (06) :1897-1901
[3]   Reactive oxygen and nitrogen intermediates increase transforming growth factor-β1 release from human epithelial alveolar cells through two different mechanisms [J].
Bellocq, A ;
Azoulay, E ;
Marullo, S ;
Flahault, A ;
Fouqueray, B ;
Philippe, C ;
Cadranel, J ;
Baud, L .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1999, 21 (01) :128-136
[4]  
CASSATELLA MA, 1989, IMMUNOLOGY, V66, P451
[5]   Does the cellular bronchoalveolar lavage fluid profile reflect the severity of sarcoidosis? [J].
Drent, M ;
Jacobs, JA ;
de Vries, J ;
Lamers, RJS ;
Liem, IH ;
Wouters, EFM .
EUROPEAN RESPIRATORY JOURNAL, 1999, 13 (06) :1338-1344
[6]   HYDROGEN-PEROXIDE RELEASE BY ALVEOLAR MACROPHAGES FROM SARCOID PATIENTS AND BY ALVEOLAR MACROPHAGES FROM NORMALS AFTER EXPOSURE TO RECOMBINANT INTERFERONS ALPHA-A, BETA, AND GAMMA AND 1,25-DIHYDROXYVITAMIN-D3 [J].
FELS, AOS ;
NATHAN, CF ;
COHN, ZA .
JOURNAL OF CLINICAL INVESTIGATION, 1987, 80 (02) :381-386
[7]   Inactivation of tissue inhibitor of metalloproteinase-1 by peroxynitrite [J].
Frears, ER ;
Zhang, Z ;
Blake, DR ;
OConnell, JP ;
Winyard, PG .
FEBS LETTERS, 1996, 381 (1-2) :21-24
[8]   Hypochlorous acid generated by myeloperoxidase modifies adjacent tryptophan and glycine residues in the catalytic domain of matrix metalloproteinase-7 (matrilysin) - An oxidative mechanism for restraining proteolytic activity during inflammation [J].
Fu, XY ;
Kassim, SY ;
Parks, WC ;
Heinecke, JW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (31) :28403-28409
[9]   Exhaled breath condensate:: methodological recommendations and unresolved questions [J].
Horvath, I ;
Hunt, J ;
Barnes, PJ ;
Alving, K ;
Antczak, A ;
Balint, B ;
Baraldi, E ;
Becher, G ;
van Beurden, WJC ;
Blomberg, A ;
Corradi, M ;
Dekhuijzen, R ;
Dweik, RA ;
Dwyer, T ;
Effros, R ;
Erzurum, S ;
Freels, J ;
Gaston, B ;
Gessner, C ;
Goldman, M ;
Greening, A ;
Ho, LP ;
Hohfeld, JM ;
Jöbsis, Q ;
Kharitonov, SA ;
Kelly, F ;
Laskowski, D ;
Lehmann, C ;
Lindstrom, A ;
Loukides, S ;
Marlin, D ;
Montuschi, P ;
Olin, AC ;
Redington, AE ;
Reinhold, P ;
van Rensen, ELJ ;
Robbins, R ;
Rubinstein, I ;
Silkoff, P ;
Smith, HJ ;
Steinhaeusser, WR ;
Teague, WG ;
Toren, K ;
Vass, G ;
Vaughan, J ;
Vogelberg, C ;
Wirtz, H .
EUROPEAN RESPIRATORY JOURNAL, 2005, 26 (03) :523-548
[10]   Influence of inflammatory mechanisms on the redox balance in interstitial lung diseases [J].
Lenz, AG ;
Hinze-Heyn, H ;
Schneider, A ;
Behr, J ;
Häussinger, K ;
Heindl, S ;
Stanzel, F ;
Maier, KL .
RESPIRATORY MEDICINE, 2004, 98 (08) :737-745
123