Role of catalase overproduction in drug resistance and virulence in Candida albicans

被引：18
|
作者：
Roman, Elvira [1 ]
Prieto, Daniel [1 ]
Martin, Ry [1 ]
Correia, Ines [1 ]
Mesa Arango, Ana Cecilia [3 ]
Alonso-Monge, Rebeca [1 ]
Zaragoza, Oscar [2 ]
Pla, Jesus [1 ]
机构：
[1] Univ Complutense Madrid, Fac Farm, Dept Microbiol 2, Plaza Ramon y Cajal S-N, E-28040 Madrid, Spain
[2] Inst Salud Carlos III, Natl Ctr Microbiol, Mycol Reference Lab, Madrid, Spain
[3] Univ Antioquia, Grp Invest Dermatol, Medellin, Colombia
来源：
FUTURE MICROBIOLOGY | 2016年 / 11卷 / 10期
关键词：
amphotericin B; Candida albicans; catalase; fluconazole; oxidative stress; OXIDATIVE-STRESS-RESPONSE; ACTIVATED PROTEIN-KINASE; FUNGAL PATHOGEN; AMPHOTERICIN-B; REACTIVE OXYGEN; TRANSCRIPTIONAL RESPONSE; MULTIDRUG-RESISTANCE; GENE; MECHANISMS; SURVIVAL;
D O I：
10.2217/fmb-2016-0067
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: To investigate the role of Cat1 overproduction in Candida albicans. Materials & methods: Strains overproducing the CAT1 gene were constructed. Results: Cells overproducing CAT1 were found to be more resistant to some oxidants and mammalian phagocytic cells. They also showed reduced intracellular reactive oxygen species generated by amphotericin B or ciclopirox olamine. CAT1 overproduction did not change the minimum inhibitory concentration of fungal cells to fungistatic or fungicidal azoles nor to amphotericin B although increased twofold the minimum inhibitory concentration to caspofungin. The role of Cat1 overproduction in virulence and colonization was also analyzed in mouse models. Conclusion: The overproduction of Cat1 protects against oxidants, phagocytes and certain antifungals at subinhibitory concentration but does not increase virulence in a systemic infection mouse model.
引用
收藏
页码：1279 / 1297
页数：19
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