Ratiometric Electrochemical Switch for Circulating Tumor DNA through Recycling Activation of Blocked DNAzymes

被引：43

作者：

Chai, Hua ^{[1
,2
]}

Tang, Yuguo ^{[2
]}

Guo, Zhenzhen ^{[2
,3
]}

Miao, Peng ^{[1
,2
]}

机构：

[1] Univ Sci & Technol China, Hefei 230026, Peoples R China

[2] Chinese Acad Sci, Suzhou Inst Biomed Engn & Technol, Suzhou 215163, Peoples R China

[3] Ji Hua Lab, Foshan 528200, Peoples R China

来源：

ANALYTICAL CHEMISTRY | 2022年 / 94卷 / 06期

关键词：

HYBRIDIZATION; PROBE;

D O I：

10.1021/acs.analchem.1c04037

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Circulating tumor DNA (ctDNA) serves as a powerful noninvasive and viable biomarker for the diagnosis of cancers. The abundance of ctDNA in patients with advanced stages is significantly higher than that in patients with early stages. Herein, a ratiometric electrochemical biosensor for the detection of ctDNA is developed by smart design of DNA probes and recycles of DNAzyme activation. The conformational variation of DNA structures leads to the changes of two types of electrochemical species. This enzyme-free sensing strategy promotes excellent amplification efficiency upon target recognition. The obtained results assure good analytical performances and a limit of detection as low as 25 aM is achieved. Additionally, this method exhibits outstanding selectivity and great application prospects in biological sample analysis.

引用

页码：2779 / 2784

页数：6

共 28 条

[1] Ultrasensitive assay of ctDNA based on DNA triangular prism and three-way junction nanostructures [J].

Chai, Hua ;

Miao, Peng .

CHINESE CHEMICAL LETTERS, 2021, 32 (02) :783-786

[2] Dual-recognition-based determination of ctDNA via the clamping function of peptide nucleic acid and terminal protection of small-molecule-linked DNA [J].

Chen, Chaohui ;

He, Rongxiang ;

Zhang, Zhengtao ;

Chen, Yong .

ANALYST, 2020, 145 (23) :7603-7608

[3] Rapid and ultrasensitive electrochemical detection of circulating tumor DNA by hybridization on the network of gold-coated magnetic nanoparticles [J].

Chen, Dongfei ;

Wu, Yanfang ;

Hoque, Sharmin ;

Tilley, Richard D. ;

Gooding, J. Justin .

CHEMICAL SCIENCE, 2021, 12 (14) :5196-5201

[4] High-Performance Ratiometric Electrochemical Method Based on the Combination of Signal Probe and Inner Reference Probe in One Hairpin-Structured DNA [J].

Deng, Chunyan ;

Pi, Xiaomei ;

Qian, Pin ;

Chen, Xiaoqing ;

Wu, Wuming ;

Xiang, Juan .

ANALYTICAL CHEMISTRY, 2017, 89 (01) :966-973

[5] Hybridization induced ion-barrier effect for the label-free and sensitive electrochemical sensing of Hepatocellular Carcinoma biomarker of miRNA-122 [J].

Gao, Feng ;

Chu, Yaru ;

Ai, Yijing ;

Yang, Weiqiang ;

Lin, Zhenyu ;

Wang, Qingxiang .

CHINESE CHEMICAL LETTERS, 2021, 32 (07) :2192-2196

[6] DNAzyme-Triggered Sol-Gel-Sol Transition of a Hydrogel Allows Target Cell Enrichment [J].

Hou, Min ;

Yin, Xiang ;

Jiang, Jianhui ;

He, Jianjun .

ACS APPLIED MATERIALS & INTERFACES, 2021, 13 (13) :15031-15039

[7] Fe-Au Nanoparticle-Coupling for Ultrasensitive Detections of Circulating Tumor DNA [J].

Hu, Ping ;

Zhang, Shengjian ;

Wu, Tong ;

Ni, Dalong ;

Fan, Wenpei ;

Zhu, Yan ;

Qian, Rong ;

Shi, Jianlin .

ADVANCED MATERIALS, 2018, 30 (31)

[8] DNA-catalyzed efficient production of single-stranded DNA nanostructures [J].

Jia, Youli ;

Chen, Liman ;

Liu, Jin ;

Li, Wei ;

Gu, Hongzhou .

CHEM, 2021, 7 (04) :959-981

[9] An impedimetric biosensor for E. coli O157:H7 based on the use ofself-assembled gold nanoparticles and protein G [J].

Lin, Donghai ;

Pillai, Rajesh G. ;

Lee, William Edward ;

Jemere, Abebaw B. .

MICROCHIMICA ACTA, 2019, 186 (03)

[10] Graphitic Carbon Nitride Nanosheets-Based Ratiometric Fluorescent Probe for Highly Sensitive Detection of H2O2 and Glucose [J].

Liu, Jin-Wen ;

Luo, Ying ;

Wang, Yu-Min ;

Duan, Lu-Ying ;

Jiang, Jian-Hui ;

Yu, Ru-Qin .

ACS APPLIED MATERIALS & INTERFACES, 2016, 8 (49) :33439-33445

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