Some considerations regarding the In Vitro culture of Rhodiola rosea L.
被引:0
作者:
Ghiorghita, Gogu
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机构:
Univ Bacau, Bacau, RomaniaUniv Bacau, Bacau, Romania
Ghiorghita, Gogu
[1
]
Hartan, Mihaela
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机构:
Univ Bacau, Bacau, RomaniaUniv Bacau, Bacau, Romania
Hartan, Mihaela
[1
]
Maftei, Diana-Elena
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机构:
Univ Bacau, Bacau, RomaniaUniv Bacau, Bacau, Romania
Maftei, Diana-Elena
[1
]
Nicuta, Daniela
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机构:
Univ Bacau, Bacau, RomaniaUniv Bacau, Bacau, Romania
Nicuta, Daniela
[1
]
机构:
[1] Univ Bacau, Bacau, Romania
来源:
ROMANIAN BIOTECHNOLOGICAL LETTERS
|
2011年
/
16卷
/
01期
关键词:
Rhodiola rosea;
in vitro culture;
morphogenetic reaction;
micropropagation;
D O I:
暂无
中图分类号:
Q81 [生物工程学(生物技术)];
Q93 [微生物学];
学科分类号:
071005 ;
0836 ;
090102 ;
100705 ;
摘要:
In vitro cultures of Rhodiola rosea were initiated and the morphogenetic reaction of several types of explants was tested in view of elaborating a micropropagation technique for this species, to provide in vitro regenerants for repopulating native habitats of R. rosea, where it has extinguished or is endangered It was ascertained that the most efficient variants of MS medium to provide neoplantlets from shoot nodes and apices are: N (2.0 mg l(-1) NAA), followed by hormone free MS, then KN (1 mg l(-1) Kin + 0.5 mg l(-1) NAA), and AZ (0.2 mg l(-1) IAA + 2 mg l(-1) zeatin). The in vitro regenerants transfer on Ceahlau Mountains (at 1750 meters altitude) proved that the achievement of our goal is possible.